Fast, Label-Free Force Spectroscopy of Histone–DNA Interactions in Individual Nucleosomes Using Nanopores

Herein we report a novel approach for fast, label-free probing of DNA–histone interactions in individual nucleosomes. We use solid-state nanopores to unravel individual DNA/histone complexes for the first time and find that the unraveling time depends on the applied electrophoretic force, and our re...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 135; no. 41; pp. 15350 - 15352
Main Authors Ivankin, Andrey, Carson, Spencer, Kinney, Shannon R. M, Wanunu, Meni
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 16.10.2013
Subjects
DNA - chemistry
Histones - chemistry
Models, Molecular
Nanopores
Nucleosomes - chemistry
Particle Size
Spectrum Analysis - methods
Surface Properties
Time Factors
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Summary:Herein we report a novel approach for fast, label-free probing of DNA–histone interactions in individual nucleosomes. We use solid-state nanopores to unravel individual DNA/histone complexes for the first time and find that the unraveling time depends on the applied electrophoretic force, and our results are in line with previous studies that employ optical tweezers. Our approach for studying nucleosomal interactions can greatly accelerate the understanding of fundamental mechanisms by which transcription, replication, and repair processes in a cell are modulated through DNA–histone interactions, as well as in diagnosis of diseases with abnormal patterns of DNA and histone modifications.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja408354s