Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold Nanoparticles

Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 132; no. 8; pp. 2642 - 2645
Main Authors Ghosh, Partha, Yang, Xiaochao, Arvizo, Rochelle, Zhu, Zheng-Jiang, Agasti, Sarit S, Mo, Zhihong, Rotello, Vincent M
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 03.03.2010
Subjects
Animals
beta-Galactosidase - administration & dosage
beta-Galactosidase - metabolism
Cell Death
Cell Line
Cell Membrane Permeability
Gold - chemistry
HeLa Cells
Humans
Nanoparticles - chemistry
Peptides - chemistry
Peptides - metabolism
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Summary:Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja907887z