Surface-Enhanced Raman Gene Probe for HIV Detection

• Published in: Analytical chemistry (Washington) Vol. 70; no. 7; pp. 1352 - 1356
• Main Authors: Isola, Narayana R, Stokes, David L, Vo-Dinh, Tuan
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.04.1998
• Subjects: AIDS/HIV; Base Sequence; Biological and medical sciences; Biotechnology; Chemistry; Deoxyribonucleic acid; DNA; DNA Primers; DNA Probes - biosynthesis; DNA Probes - chemistry; DNA, Viral - analysis; Fundamental and applied biological sciences. Psychology; Genes; Genes, gag - genetics; Genetic engineering; Genetic technics; HIV; HIV - genetics; HIV - isolation & purification; Human immunodeficiency virus; Humans; In vitro gene amplification. Pcr technique; Methods. Procedures. Technologies; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction - methods; Spectrum Analysis, Raman - instrumentation; Spectrum Analysis, Raman - methods; Surface Properties; Virus; Performance evaluation; Polymerase chain reaction; Retroviridae; Model study; Molecular probe; Raman spectrometry; Chemical labelling; Human immunodeficiency virus; Lentivirus
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac970901z

We report, for the first time, the use of surface-enhanced Raman (SERS)-active labels for primers used in polymerase chain reaction amplification of specific target DNA sequences. This method has the potential for combining the spectral selectivity and high sensitivity of the SERS technique with the inherent molecular specificity offered by DNA sequence hybridization. The effectiveness of the detection scheme is demonstrated using the gag gene sequence of the human immunodeficiency virus. The potential use of multiple probes for simultaneous detection of multiple biological targets is discussed.