An Allosteric Dual-DNAzyme Unimolecular Probe for Colorimetric Detection of Copper(II)

An effective dual-DNAzyme-based unimolecular probe design employing intramolecular signal transduction is demonstrated. The probe is composed of three domains: a DNA-cleaving DNAzyme, a substrate, and an HRP-mimicking DNAzyme. When the probe meets its target, cleavage of the substrate by the DNA-cle...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 131; no. 41; pp. 14624 - 14625
Main Authors Yin, Bin-Cheng, Ye, Bang-Ce, Tan, Weihong, Wang, Hui, Xie, Cong-Cong
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 21.10.2009
Subjects
Allosteric Regulation
Base Sequence
Biosensing Techniques - methods
Colorimetry
Copper - analysis
Copper - metabolism
DNA, Catalytic - genetics
DNA, Catalytic - metabolism
Horseradish Peroxidase - metabolism
Humans
Protein Engineering
Online AccessGet full text

Cover

More Information
Summary:An effective dual-DNAzyme-based unimolecular probe design employing intramolecular signal transduction is demonstrated. The probe is composed of three domains: a DNA-cleaving DNAzyme, a substrate, and an HRP-mimicking DNAzyme. When the probe meets its target, cleavage of the substrate by the DNA-cleaving DNAzyme activates the HRP-mimicking DNAzyme, producing a colorimetric signal. The Cu2+-dependent DNAzyme engineered to demonstrate this design revealed a sensitivity corresponding to 65 ppb, which is sufficient to detect Cu2+ in drinking water. The new probe has excellent selectivity toward Cu2+. This three-component design is simple and easy to engineer. It may provide the basis for future development of other nucleic acid-based probes for toxicological and environmental monitoring.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja9062426