Elucidation of the Molecular Basis of Cholecystokinin Peptide Docking to Its Receptor Using Site-Specific Intrinsic Photoaffinity Labeling and Molecular Modeling

• Published in: Biochemistry (Easton) Vol. 48; no. 23; pp. 5303 - 5312
• Main Authors: Dong, Maoqing, Lam, Polo C.-H, Pinon, Delia I, Abagyan, Ruben, Miller, Laurence J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.06.2009
• Subjects: Amino Acid Sequence; Animals; Binding Sites; Cercopithecus aethiops; CHO Cells; Cholecystokinin - chemistry; Cholecystokinin - metabolism; COS Cells; Cricetinae; Cricetulus; Fluorescence Resonance Energy Transfer; Models, Molecular; Molecular Sequence Data; Peptides - chemistry; Peptides - metabolism; Photoaffinity Labels - chemistry; Protein Conformation; Receptor, Adenosine A2A - chemistry; Receptor, Adenosine A2A - metabolism; Receptors, Adrenergic, beta-2 - chemistry; Receptors, Adrenergic, beta-2 - metabolism; Receptors, Cholecystokinin - chemistry; Receptors, Cholecystokinin - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi9004705

G protein-coupled receptors represent the largest family of receptors and the major target of current drug development efforts. Understanding of the mechanisms of ligand binding and activation of these receptors remains limited, despite recent advances in structural determination of family members. This work focuses on the use of photoaffinity labeling and molecular modeling to elucidate the structural basis of binding a natural peptide ligand to a family A G protein-coupled receptor, the type 1 cholecystokinin receptor. Two photolabile cholecystokinin analogues were developed and characterized as representing high-affinity, fully biologically active probes with sites of covalent attachment at positions 28 and 31. The sites of receptor labeling were identified by purification, proteolytic peptide mapping, and radiochemical sequencing of labeled wild-type and mutant cholecystokinin receptors. The position 28 probe labeled second extracellular loop residue Leu199, while the position 31 probe labeled first extracellular loop residue Phe107. Along with five additional spatial approximation constraints coming from previous photoaffinity labeling studies and 12 distance restraints from fluorescence resonance energy transfer studies, these were built into two homology models of the cholecystokinin receptor, based on the recent crystal structures of the β2-adrenergic receptor and A2a-adenosine receptor. The resultant agonist ligand-occupied receptor models fully accommodate all existing experimental data and represent the best refined models of a peptide hormone receptor in this important family.