Disposition of Flavonoids via Enteric Recycling: Enzyme Stability Affects Characterization of Prunetin Glucuronidation across Species, Organs, and UGT Isoforms

• Published in: Molecular pharmaceutics Vol. 4; no. 6; pp. 883 - 894
• Main Authors: Joseph, Tiby B, Wang, Stephen W. J, Liu, Xing, Kulkarni, Kaustubh H, Wang, Jingrong, Xu, Haiyan, Hu, Ming
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.11.2007
• Subjects: Animals; Antineoplastic Agents; Enzyme Stability; Flavonoids - metabolism; Glucuronides - metabolism; Glucuronosyltransferase - metabolism; Hot Temperature; Humans; Intestines; Isoflavones - metabolism; Liver; Metabolic Networks and Pathways; Mice; Microsomes - metabolism; Organ Specificity; Phytoestrogens - metabolism; Protein Isoforms - metabolism; Rats; Species Specificity; prunetin; isoflavone; Glucuronidation; UGT; bioavailability; flavonoids; stability
• ISSN: 1543-8384; 1543-8392
• DOI: 10.1021/mp700135a

We characterized the in vitro glucuronidation of prunetin, a prodrug of genistein that is a highly active cancer prevention agent. Metabolism studies were conducted using expressed human UGT isoforms and microsomes/S9 fractions prepared from intestine and liver of rodents and humans. The results indicated that human intestinal microsomes were more efficient than liver microsomes in glucuronidating prunetin, but rates of metabolism were dependent on time of incubation at 37 °C. Human liver and intestinal microsomes mainly produced metabolite 1 (prunetin-5-O-glucuronide) and metabolite 2 (prunetin-4′-O-glucuronide), respectively. Using 12 human UGT isoforms, we showed that UGT1A7, UGT1A8, and UGT1A9 were mainly responsible for the formation of metabolite 1, whereas UGT1A1, UGT1A8, and UGT1A10 were mainly responsible for the formation of metabolite 2. This isoform-specific metabolism was consistent with earlier results obtained using human liver and intestinal microsomes, as the former (liver) is UGT1A9-rich whereas the latter is UGT1A10-rich. Surprisingly, we found that the thermostability of the microsomes was isoform- and organ-dependent. For example, human liver microsomal UGT activities were much more heat-stable (37 °C) than intestinal microsomal UGT activities, consistent with the finding that human UGT1A9 is much more thermostable than human UGT1A10 and UGT1A8. The organ-specific thermostability profiles were also evident in rat microsomes and mouse S9 fractions, even though human intestinal glucuronidation of prunetin differs significantly from rodent intestinal glucuronidation. In conclusion, prunetin glucuronidation is species-, organ-, and UGT-isoform-dependent, all of which may be impacted by the thermostability of specific UGT isoforms involved in the metabolism.