3‑D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening

The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being indu...

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Bibliographic Details
Published inACS biomaterials science & engineering Vol. 6; no. 12; pp. 6701 - 6709
Main Authors Guo, Haibin, Deng, Nan, Dou, Lei, Ding, Huifen, Criswell, Tracy, Atala, Anthony, Furdui, Cristina M, Zhang, Yuanyuan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 14.12.2020
Subjects
Cell Differentiation
Cisplatin
Humans
Kidney
Modeling and Informatics Tools
Organoids
Stem Cells
drug testing
3d culture
renal progenitor cells
kidney extracellular matrix
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Summary:The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.
ISSN:2373-9878
2373-9878
DOI:10.1021/acsbiomaterials.0c01468