Utilizing Recombinant Spider Silk Proteins To Develop a Synthetic Bruch’s Membrane for Modeling the Retinal Pigment Epithelium

Spider silks are intriguing biomaterials that have a high potential as innovative biomedical processes and devices. The intent of this study was to evaluate the capacity of recombinant spider silk proteins (rSSps) as a synthetic Bruch’s membrane. Nonporous silk membranes were prepared with comparabl...

Full description

Saved in:
Bibliographic Details
Published inACS biomaterials science & engineering Vol. 5; no. 8; pp. 4023 - 4036
Main Authors Harris, Thomas I, Paterson, Chase A, Farjood, Farhad, Wadsworth, Ian D, Caldwell, Lori, Lewis, Randolph V, Jones, Justin A, Vargis, Elizabeth
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 12.08.2019
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Spider silks are intriguing biomaterials that have a high potential as innovative biomedical processes and devices. The intent of this study was to evaluate the capacity of recombinant spider silk proteins (rSSps) as a synthetic Bruch’s membrane. Nonporous silk membranes were prepared with comparable thicknesses (<10 μm) to that of native Bruch’s membrane. Biomechanical characterization was performed prior to seeding cells. The ability of RPE cells (ARPE-19) to attach and grow on the membranes was then evaluated with bright-field and electron microscopy, intracellular DNA quantification, and immunocytochemical staining (ZO-1 and F-actin). Controls were cultured on permeable Transwell support membranes and characterized with the same methods. A size-dependent permeability assay, using FITC–dextran, was used to determine cell-membrane barrier function. Compared to Transwell controls, RPE cells cultured on rSSps membranes developed more native-like “cobblestone” morphologies, exhibited higher intracellular DNA content, and expressed key organizational proteins more consistently. Comparisons of the membranes to native structures revealed that the silk membranes exhibited equivalent thicknesses, biomechanical properties, and barrier functions. These findings support the use of recombinant spider silk proteins to model Bruch’s membrane and develop more biomimetic retinal models.
AbstractList Spider silks are intriguing biomaterials that have a high potential as innovative biomedical processes and devices. The intent of this study was to evaluate the capacity of recombinant spider silk proteins (rSSps) as a synthetic Bruch's membrane. Nonporous silk membranes were prepared with comparable thicknesses (<10 μm) to that of native Bruch's membrane. Biomechanical characterization was performed prior to seeding cells. The ability of RPE cells (ARPE-19) to attach and grow on the membranes was then evaluated with bright-field and electron microscopy, intracellular DNA quantification, and immunocytochemical staining (ZO-1 and F-actin). Controls were cultured on permeable Transwell support membranes and characterized with the same methods. A size-dependent permeability assay, using FITC-dextran, was used to determine cell-membrane barrier function. Compared to Transwell controls, RPE cells cultured on rSSps membranes developed more native-like "cobblestone" morphologies, exhibited higher intracellular DNA content, and expressed key organizational proteins more consistently. Comparisons of the membranes to native structures revealed that the silk membranes exhibited equivalent thicknesses, biomechanical properties, and barrier functions. These findings support the use of recombinant spider silk proteins to model Bruch's membrane and develop more biomimetic retinal models.
Spider silks are intriguing biomaterials that have a high potential as innovative biomedical processes and devices. The intent of this study was to evaluate the capacity of recombinant spider silk proteins (rSSps) as a synthetic Bruch’s membrane. Nonporous silk membranes were prepared with comparable thicknesses (<10 μ m) to that of native Bruch’s membrane. Biomechanical characterization was performed prior to seeding cells. The ability of RPE cells (ARPE-19) to attach and grow on the membranes was then evaluated with bright-field and electron microscopy, intracellular DNA quantification, and immunocyto-chemical staining (ZO-1 and F-actin). Controls were cultured on permeable Transwell support membranes and characterized with the same methods. A size-dependent permeability assay, using FITC–dextran, was used to determine cell-membrane barrier function. Compared to Transwell controls, RPE cells cultured on rSSps membranes developed more native-like “cobblestone” morphologies, exhibited higher intracellular DNA content, and expressed key organizational proteins more consistently. Comparisons of the membranes to native structures revealed that the silk membranes exhibited equivalent thicknesses, biomechanical properties, and barrier functions. These findings support the use of recombinant spider silk proteins to model Bruch’s membrane and develop more biomimetic retinal models.
Author Vargis, Elizabeth
Wadsworth, Ian D
Jones, Justin A
Caldwell, Lori
Harris, Thomas I
Lewis, Randolph V
Paterson, Chase A
Farjood, Farhad
AuthorAffiliation Departments of Biological Engineering
Biology
AuthorAffiliation_xml – name: Departments of Biological Engineering
– name: Biology
– name:
– name: Department of Biology, Utah State University, Logan, Utah 84322, United States
– name: Department of Biological Engineering, Utah State University, Logan, Utah 84322, United States
Author_xml – sequence: 1
  givenname: Thomas I
  orcidid: 0000-0001-6575-2551
  surname: Harris
  fullname: Harris, Thomas I
– sequence: 2
  givenname: Chase A
  orcidid: 0000-0002-4522-437X
  surname: Paterson
  fullname: Paterson, Chase A
– sequence: 3
  givenname: Farhad
  orcidid: 0000-0002-8826-6721
  surname: Farjood
  fullname: Farjood, Farhad
– sequence: 4
  givenname: Ian D
  orcidid: 0000-0002-0345-0598
  surname: Wadsworth
  fullname: Wadsworth, Ian D
– sequence: 5
  givenname: Lori
  orcidid: 0000-0002-8308-7548
  surname: Caldwell
  fullname: Caldwell, Lori
– sequence: 6
  givenname: Randolph V
  surname: Lewis
  fullname: Lewis, Randolph V
– sequence: 7
  givenname: Justin A
  orcidid: 0000-0002-3647-5361
  surname: Jones
  fullname: Jones, Justin A
  email: justin.a.jones@usu.edu
– sequence: 8
  givenname: Elizabeth
  orcidid: 0000-0003-3141-9317
  surname: Vargis
  fullname: Vargis, Elizabeth
  email: vargis@usu.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33448804$$D View this record in MEDLINE/PubMed
BookMark eNqFkctO3DAUhq2KqlDgFVovuxnq2J7E2VRqKb1IoCIG1pbjHM-YOnawHSS64jX6en2SejQDgm668u07n8_R_xrt-OABobcVOaoIrd4rnTobBpUhWuXSUdsRUgn2Au1R1rBZKxqx82S_iw5TuiaFYWLOOX-FdhnjXAjC99D9VbbO_rJ-iS9Ah6GzXvmMF6PtIeKFdT_xeQwZrE_4MuDPcAsujFjhxZ3PK8hW409x0qs_978TPoOhi8oDNiHis9CDW3sLVty5iB0-t8sBiv9ktOXa2Wk4QC9NGQIOt-s-uvpycnn8bXb64-v344-nM8XrKs_mWtWGdrrnNemMVm3VCAYt1eXYm26uOsFMY3TfN8C1gUrTXtOaVbUA2rbA9tGHjXecugF6XbqIyskx2kHFOxmUlc9fvF3JZbiVgjRiLmgRvNsKYriZIGU52KTBuTJwmJKkfM3xhoqCNhtUx5BSBPP4TUXkOkL5T4RyG2GpfPO0y8e6h8AKwDZAMcjrMEW_Lv-f9i_uNLXL
CitedBy_id crossref_primary_10_1021_acsbiomaterials_3c00411
crossref_primary_10_3389_fbioe_2022_958486
crossref_primary_10_4103_NRR_NRR_D_23_01789
crossref_primary_10_1021_acsbiomaterials_1c00612
crossref_primary_10_3389_fbioe_2020_588014
crossref_primary_10_1002_adfm_202002982
crossref_primary_10_1177_08853282211003850
crossref_primary_10_3390_biomedicines12050966
crossref_primary_10_1097_APO_0000000000000521
Cites_doi 10.1136/bjo.2009.171926
10.1002/jbm.a.35690
10.1016/j.mam.2012.04.005
10.1016/S1350-9462(02)00043-5
10.1016/j.biomaterials.2012.02.040
10.1002/(SICI)1097-4636(199701)34:1<87::AID-JBM12>3.0.CO;2-M
10.1002/bip.21715
10.1152/physrev.00021.2004
10.1136/bjo.2009.166728
10.1007/s11095-018-2534-y
10.1016/S0161-6420(90)32619-2
10.1016/S0140-6736(06)69740-7
10.1016/j.biomaterials.2006.11.023
10.1016/j.preteyeres.2015.06.002
10.1016/j.bpj.2010.12.3744
10.1186/s12866-015-0408-6
10.1089/ten.tea.2007.0224
10.1002/app.10366
10.1167/iovs.04-1051
10.1016/j.molmed.2015.12.007
10.1088/1758-5082/5/4/045008
10.1016/j.matbio.2015.10.003
10.1016/j.copbio.2012.03.013
10.1242/jcs.1993.Supplement_17.27
10.1021/acsomega.7b01604
10.1042/BA20090229
10.3390/ma2041908
10.1016/S0140-6736(12)60028-2
10.1007/s10456-007-9067-z
10.1007/s00253-015-6948-8
10.1016/j.biomaterials.2012.05.069
10.1021/acsami.7b10874
10.1016/j.jbiomech.2014.06.021
10.1136/bmj.326.7387.485
10.1002/jbm.a.34178
10.1021/bm5007823
10.1001/archopht.1964.00970020380016
10.1016/S0074-7696(07)58004-6
10.1007/s10544-012-9645-8
10.3390/ijms10041514
10.1016/j.biomaterials.2015.01.044
10.1016/j.jmbbm.2013.09.002
10.1136/bjo.2009.176305
10.1111/j.1742-4658.2007.05823.x
10.1016/j.biomaterials.2004.08.022
10.1002/bip.21724
10.1167/iovs.03-0943
10.1128/AEM.01120-17
10.1002/cm.20041
10.1007/s00417-009-1263-7
10.1038/eye.2001.141
10.1016/j.biomaterials.2010.08.061
10.1016/j.exer.2014.02.020
10.1290/1071-2690(2002)038<0228:AIVMOT>2.0.CO;2
10.1016/B978-1-4557-0737-9.00020-5
10.1002/jbm.b.31875
10.1021/bm301110s
10.1002/stem.635
10.1016/j.preteyeres.2009.08.003
10.1021/acsami.8b05853
10.1016/j.jneumeth.2011.05.012
10.1021/acs.biomac.5b00226
10.1016/S0141-8130(98)00089-0
10.1083/jcb.42.2.392
10.1016/S0021-9258(18)41777-2
10.1056/NEJMra062326
10.1016/S1350-9462(97)00012-8
10.1136/bjo.2010.184002
10.1002/term.2089
10.1016/S0142-9612(02)00353-8
10.1016/S0076-6879(06)18011-8
10.1002/term.1458
10.1021/acs.biomac.6b01267
10.2147/OPTH.S2151
10.1016/B978-0-12-407679-2.00004-1
10.1021/cr010194g
10.1016/j.biomaterials.2011.02.040
10.1038/eye.1987.35
10.1016/S0142-9612(01)00171-5
10.1016/j.biomaterials.2010.12.041
10.1097/00006982-199414020-00006
10.1016/j.biomaterials.2014.08.040
10.1007/s00018-010-0462-z
10.1016/j.biomaterials.2013.07.058
10.1016/S0002-9394(14)73079-X
ContentType Journal Article
DBID NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1021/acsbiomaterials.9b00183
DatabaseName PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle PubMed
CrossRef
MEDLINE - Academic
DatabaseTitleList PubMed


Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
EISSN 2373-9878
EndPage 4036
ExternalDocumentID 10_1021_acsbiomaterials_9b00183
33448804
d44188269
Genre Journal Article
GrantInformation_xml – fundername: NEI NIH HHS
  grantid: R15 EY028732
GroupedDBID ABMVS
ABUCX
ACGFS
ACS
AEESW
AFEFF
ALMA_UNASSIGNED_HOLDINGS
AQSVZ
EBS
EJD
UI2
VF5
VG9
W1F
53G
ABQRX
ADHLV
AHGAQ
BAANH
CUPRZ
GGK
NPM
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-a461t-5ca6f2bcd460bfca91783e92c60bdfb5ab83f7fcdd7e4cfe1c2dc263168e299e3
IEDL.DBID ACS
ISSN 2373-9878
IngestDate Tue Sep 17 21:27:19 EDT 2024
Fri Aug 16 04:27:45 EDT 2024
Fri Dec 06 00:58:22 EST 2024
Sat Sep 28 08:23:22 EDT 2024
Thu Aug 27 13:43:30 EDT 2020
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed false
IsScholarly true
Issue 8
Keywords films
Bruch’s membrane
scaffold
biomimetic
model
spider silk
RPE cells
Language English
License https://doi.org/10.15223/policy-029
https://doi.org/10.15223/policy-037
https://doi.org/10.15223/policy-045
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-a461t-5ca6f2bcd460bfca91783e92c60bdfb5ab83f7fcdd7e4cfe1c2dc263168e299e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Author Contributions
The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript.
ORCID 0000-0001-6575-2551
0000-0002-4522-437X
0000-0002-8826-6721
0000-0002-3647-5361
0000-0002-0345-0598
0000-0002-8308-7548
0000-0003-3141-9317
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078582
PMID 33448804
PQID 2478584728
PQPubID 23479
PageCount 14
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_8078582
proquest_miscellaneous_2478584728
crossref_primary_10_1021_acsbiomaterials_9b00183
pubmed_primary_33448804
acs_journals_10_1021_acsbiomaterials_9b00183
ProviderPackageCode ACS
AEESW
AFEFF
VF5
VG9
ABMVS
ABUCX
AQSVZ
W1F
UI2
PublicationCentury 2000
PublicationDate 2019-08-12
PublicationDateYYYYMMDD 2019-08-12
PublicationDate_xml – month: 08
  year: 2019
  text: 2019-08-12
  day: 12
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle ACS biomaterials science & engineering
PublicationTitleAlternate ACS Biomater. Sci. Eng
PublicationYear 2019
Publisher American Chemical Society
Publisher_xml – name: American Chemical Society
References ref9/cit9
ref45/cit45
ref3/cit3
ref27/cit27
ref81/cit81
ref63/cit63
ref56/cit56
ref16/cit16
Farjood F. (ref72/cit72) 2017; 23
Hinman M. B. (ref43/cit43) 1992; 267
ref52/cit52
ref23/cit23
ref8/cit8
ref31/cit31
ref59/cit59
ref85/cit85
ref2/cit2
ref77/cit77
ref34/cit34
ref71/cit71
ref37/cit37
ref20/cit20
ref48/cit48
ref60/cit60
ref74/cit74
ref88/cit88
ref17/cit17
ref82/cit82
ref10/cit10
ref35/cit35
ref53/cit53
ref19/cit19
ref21/cit21
ref42/cit42
Chan W. H. (ref73/cit73) 2007; 48
ref46/cit46
ref49/cit49
ref13/cit13
ref61/cit61
ref75/cit75
ref67/cit67
ref24/cit24
ref38/cit38
ref50/cit50
ref64/cit64
ref78/cit78
ref54/cit54
ref6/cit6
ref36/cit36
ref18/cit18
ref83/cit83
ref65/cit65
ref79/cit79
ref11/cit11
ref25/cit25
ref29/cit29
ref76/cit76
ref86/cit86
ref32/cit32
ref39/cit39
ref14/cit14
ref57/cit57
ref5/cit5
ref51/cit51
ref80/cit80
ref28/cit28
ref40/cit40
ref68/cit68
Burns R. P. (ref7/cit7) 1980; 78
ref26/cit26
ref55/cit55
ref69/cit69
ref12/cit12
ref15/cit15
ref62/cit62
ref66/cit66
ref41/cit41
ref58/cit58
ref22/cit22
ref33/cit33
ref87/cit87
ref4/cit4
ref30/cit30
ref47/cit47
ref84/cit84
ref1/cit1
ref44/cit44
ref70/cit70
References_xml – ident: ref23/cit23
  doi: 10.1136/bjo.2009.171926
– ident: ref33/cit33
  doi: 10.1002/jbm.a.35690
– ident: ref4/cit4
  doi: 10.1016/j.mam.2012.04.005
– ident: ref6/cit6
  doi: 10.1016/S1350-9462(02)00043-5
– ident: ref53/cit53
  doi: 10.1016/j.biomaterials.2012.02.040
– ident: ref30/cit30
  doi: 10.1002/(SICI)1097-4636(199701)34:1<87::AID-JBM12>3.0.CO;2-M
– ident: ref38/cit38
  doi: 10.1002/bip.21715
– ident: ref11/cit11
  doi: 10.1152/physrev.00021.2004
– ident: ref26/cit26
  doi: 10.1136/bjo.2009.166728
– ident: ref61/cit61
  doi: 10.1007/s11095-018-2534-y
– ident: ref5/cit5
  doi: 10.1016/S0161-6420(90)32619-2
– ident: ref19/cit19
  doi: 10.1016/S0140-6736(06)69740-7
– ident: ref80/cit80
  doi: 10.1016/j.biomaterials.2006.11.023
– ident: ref10/cit10
  doi: 10.1016/j.preteyeres.2015.06.002
– ident: ref86/cit86
  doi: 10.1016/j.bpj.2010.12.3744
– ident: ref66/cit66
  doi: 10.1186/s12866-015-0408-6
– ident: ref55/cit55
  doi: 10.1089/ten.tea.2007.0224
– ident: ref58/cit58
  doi: 10.1002/app.10366
– ident: ref77/cit77
  doi: 10.1167/iovs.04-1051
– ident: ref27/cit27
  doi: 10.1016/j.molmed.2015.12.007
– ident: ref63/cit63
  doi: 10.1088/1758-5082/5/4/045008
– ident: ref64/cit64
  doi: 10.1016/j.matbio.2015.10.003
– ident: ref46/cit46
  doi: 10.1016/j.copbio.2012.03.013
– ident: ref16/cit16
  doi: 10.1242/jcs.1993.Supplement_17.27
– ident: ref34/cit34
  doi: 10.1021/acsomega.7b01604
– ident: ref37/cit37
  doi: 10.1042/BA20090229
– ident: ref40/cit40
  doi: 10.3390/ma2041908
– ident: ref22/cit22
  doi: 10.1016/S0140-6736(12)60028-2
– ident: ref79/cit79
  doi: 10.1007/s10456-007-9067-z
– volume: 23
  start-page: 431
  year: 2017
  ident: ref72/cit72
  publication-title: Mol. Vis.
  contributor:
    fullname: Farjood F.
– ident: ref84/cit84
  doi: 10.1007/s00253-015-6948-8
– ident: ref52/cit52
  doi: 10.1016/j.biomaterials.2012.05.069
– volume: 48
  start-page: 2187
  year: 2007
  ident: ref73/cit73
  publication-title: Invest. Ophthalmol. Vis. Sci.
  contributor:
    fullname: Chan W. H.
– ident: ref60/cit60
  doi: 10.1021/acsami.7b10874
– ident: ref87/cit87
  doi: 10.1016/j.jbiomech.2014.06.021
– ident: ref17/cit17
  doi: 10.1136/bmj.326.7387.485
– ident: ref29/cit29
  doi: 10.1002/jbm.a.34178
– ident: ref67/cit67
  doi: 10.1021/bm5007823
– ident: ref2/cit2
  doi: 10.1001/archopht.1964.00970020380016
– ident: ref78/cit78
  doi: 10.1016/S0074-7696(07)58004-6
– ident: ref32/cit32
  doi: 10.1007/s10544-012-9645-8
– ident: ref39/cit39
  doi: 10.3390/ijms10041514
– ident: ref48/cit48
  doi: 10.1016/j.biomaterials.2015.01.044
– ident: ref70/cit70
  doi: 10.1016/j.jmbbm.2013.09.002
– ident: ref24/cit24
  doi: 10.1136/bjo.2009.176305
– ident: ref74/cit74
  doi: 10.1111/j.1742-4658.2007.05823.x
– ident: ref31/cit31
  doi: 10.1016/j.biomaterials.2004.08.022
– ident: ref83/cit83
  doi: 10.1002/bip.21724
– ident: ref88/cit88
  doi: 10.1167/iovs.03-0943
– ident: ref71/cit71
  doi: 10.1128/AEM.01120-17
– ident: ref85/cit85
  doi: 10.1002/cm.20041
– ident: ref25/cit25
  doi: 10.1007/s00417-009-1263-7
– ident: ref15/cit15
  doi: 10.1038/eye.2001.141
– ident: ref50/cit50
  doi: 10.1016/j.biomaterials.2010.08.061
– ident: ref76/cit76
  doi: 10.1016/j.exer.2014.02.020
– ident: ref62/cit62
  doi: 10.1290/1071-2690(2002)038<0228:AIVMOT>2.0.CO;2
– ident: ref1/cit1
  doi: 10.1016/B978-1-4557-0737-9.00020-5
– ident: ref56/cit56
  doi: 10.1002/jbm.b.31875
– ident: ref82/cit82
  doi: 10.1021/bm301110s
– ident: ref14/cit14
  doi: 10.1002/stem.635
– ident: ref3/cit3
  doi: 10.1016/j.preteyeres.2009.08.003
– ident: ref59/cit59
  doi: 10.1021/acsami.8b05853
– ident: ref65/cit65
  doi: 10.1016/j.jneumeth.2011.05.012
– ident: ref68/cit68
  doi: 10.1021/acs.biomac.5b00226
– ident: ref45/cit45
  doi: 10.1016/S0141-8130(98)00089-0
– ident: ref13/cit13
  doi: 10.1083/jcb.42.2.392
– volume: 267
  start-page: 19320
  year: 1992
  ident: ref43/cit43
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)41777-2
  contributor:
    fullname: Hinman M. B.
– ident: ref18/cit18
  doi: 10.1056/NEJMra062326
– ident: ref20/cit20
  doi: 10.1016/S1350-9462(97)00012-8
– ident: ref81/cit81
  doi: 10.1136/bjo.2010.184002
– volume: 78
  start-page: 206
  year: 1980
  ident: ref7/cit7
  publication-title: Trans. Am. Ophthalmol. Soc.
  contributor:
    fullname: Burns R. P.
– ident: ref54/cit54
  doi: 10.1002/term.2089
– ident: ref57/cit57
  doi: 10.1016/S0142-9612(02)00353-8
– ident: ref12/cit12
  doi: 10.1016/S0076-6879(06)18011-8
– ident: ref36/cit36
  doi: 10.1002/term.1458
– ident: ref69/cit69
  doi: 10.1021/acs.biomac.6b01267
– ident: ref9/cit9
  doi: 10.2147/OPTH.S2151
– ident: ref47/cit47
  doi: 10.1016/B978-0-12-407679-2.00004-1
– ident: ref44/cit44
  doi: 10.1021/cr010194g
– ident: ref49/cit49
  doi: 10.1016/j.biomaterials.2011.02.040
– ident: ref75/cit75
  doi: 10.1038/eye.1987.35
– ident: ref28/cit28
  doi: 10.1016/S0142-9612(01)00171-5
– ident: ref42/cit42
  doi: 10.1016/j.biomaterials.2010.12.041
– ident: ref8/cit8
  doi: 10.1097/00006982-199414020-00006
– ident: ref35/cit35
  doi: 10.1016/j.biomaterials.2014.08.040
– ident: ref41/cit41
  doi: 10.1007/s00018-010-0462-z
– ident: ref51/cit51
  doi: 10.1016/j.biomaterials.2013.07.058
– ident: ref21/cit21
  doi: 10.1016/S0002-9394(14)73079-X
SSID ssj0001385444
Score 2.2444892
Snippet Spider silks are intriguing biomaterials that have a high potential as innovative biomedical processes and devices. The intent of this study was to evaluate...
SourceID pubmedcentral
proquest
crossref
pubmed
acs
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 4023
Title Utilizing Recombinant Spider Silk Proteins To Develop a Synthetic Bruch’s Membrane for Modeling the Retinal Pigment Epithelium
URI http://dx.doi.org/10.1021/acsbiomaterials.9b00183
https://www.ncbi.nlm.nih.gov/pubmed/33448804
https://search.proquest.com/docview/2478584728
https://pubmed.ncbi.nlm.nih.gov/PMC8078582
Volume 5
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB6VcoEDb8rykpE4kiWxHTs5VlWrCqmoYrtSb5Ht2DTqbrZqsgd66t_g7_FLmEmypduqAm6J4kzk8TjzjecF8NGkaOqoREWZcHEkFc8ja3we-URkVPArFpqykQ--qv2p_HKcHm9AcocHnyefjWsoE920_YqMqYgfyuE9uI-UNAn19s7kz7EKfkJ2LVy50CJCizpbRXXdTYs0k2vWNdMtuHkzavKaGtp7DN9WyTx99MnpeNnasbu4Xdvx32f4BB4NoJRt91L0FDZ8_QweXitV-Bwup201qy7wmpHFOrddBA2bUIPZczapZqfskEo-VHXDjhZsiEVihk1-1IgxkTAjMTr5dfmzYQd-jkZ67RkiZkbd2CgnnuEwpN1Smy52WH2nY0u2e0Y5I7NqOX8B073do539aOjeEBlc_DZKnVGBW1dKFdvgDNqFmfA5d3hbBpsam4mggytL7aULPnG8dFxRIy2POtKLl7BZL2r_CpjBv4xGYBKUD9KVIRfOKm0FosMQx6UbwSfkYTHsvqboHOs8KW4wthgYO4J4tdDFWV_T4--vfFgJRIH7j5wqyKXFsim41Bm5mnk2gq1eQK6ICiHp_yhHoNdE52oA1fZef1JXJ12Nb2oDkGb89f_N7A08QECX05l3wt_CZnu-9O8QNLX2fbdNfgPugBru
link.rule.ids 230,314,780,784,885,2765,27076,27924,27925,56738,56788
linkProvider American Chemical Society
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDCa67rDt0L23dC8N2HHObMmW5eNQtMi2piiQBOhOhiVLq9HEKWrn0J76N_r3-ktKOk7aZBiG7eaHTFsSZX2UyI8An7IITR0ZSE8J43uh5ImnM5t4NhCKCL98EVM0cv9A9kbh96PoaAPUIhYGP6JCSVWziX_LLhB8wWsUkJ7V847pEpcfquM9uB9R4koCRTuD29UVfFPYZHLlIhYeGtZq4dz1Z1k0QZlqdYL6DXWuO0_emY32HsPPZT0aJ5ST7qzWXXOxRvH4PxV9AlstRGVf5zr1FDZs-Qwe3SEufA6Xo7oYFxd4zMh-nejGn4YNKN3sGRsU4xN2SAQQRVmx4ZS1nkksY4PzEhEnCmakVMfXl1cV69sJmuylZYifGeVmowh5hsVQdk1Ju9hh8YsWMdnuKUWQjIvZ5AWM9naHOz2vzeXgZagKtReZTDquTR5KXzuToZWohE24wdPc6SjTSrjYmTyPbWicDQzPDZeUVsvijGnFS9gsp6V9DSzDf06MMMVJ60KTu0QYLWMtECs6389NBz5jG6btWKzSZpudB-law6Ztw3bAX_R3ejpn-Pj7Ix8XepHiaKQtFmyl6axKeRgr2njmqgOv5nqyFCpESH_LsAPxigYtCxDT9-qdsjhuGL8pKUCk-Pa_1ewDPOgN-_vp_reDH2_gIUK9hFbDA_4WNuuzmX2HcKrW75uRcwNltyNb
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwED-NISF4YHxTNsBIPJIusR0nkXiZtlXjY1OlrtJeUBQ7NovWptWSPrCn_Rv8e_wl3KVpWYsQgrd8OJf4cvbd2Xe_A3ibhejqqEB5sTC-JxVPPJ3ZxLOBiAnwyxcRZSMfn6ijofx4Fp5twPtFLgx-RIWUqmYTn0b1NHctwkCwi9cpKT2r5z-nS3h-KJK34HaIcy4FdO3tD36tsODbZFPNlYtIeOhcx4sArz_TIiVlqlUl9ZvluR5AeUMj9bbgy7IvTSDKRXdW6665WoN5_N_OPoD7ranK9uay9RA2bPkI7t0AMHwM18O6GBVXeMzIjx3rJq6GDajs7CUbFKML1icgiKKs2OmEtRFKLGODbyVankiYkXCd_7j-XrFjO0bXvbQM7WhGNdooU55hM6RdU_Eu1i--0mImO5xSJsmomI2fwLB3eLp_5LU1HbwMRaL2QpMpx7XJpfK1Mxl6i7GwCTd4mjsdZjoWLnImzyMrjbOB4bnhisprWdScVjyFzXJS2ufAMpx7IjRXnLJOmtwlwmgVaYE2o_P93HTgHfIwbcdklTbb7TxI1xibtoztgL_45-l0jvTx90feLGQjxVFJWy3IpcmsSrmMYtqA5nEHns1lZUlUCEmzpuxAtCJFywaE-L16pyzOG-RvKg4QxvzFv_XsNdzpH_TSzx9OPm3DXbT4EloUD_gObNaXM_sSrapav2oGz09sFyXe
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Utilizing+Recombinant+Spider+Silk+Proteins+To+Develop+a+Synthetic+Bruch%E2%80%99s+Membrane+for+Modeling+the+Retinal+Pigment+Epithelium&rft.jtitle=ACS+biomaterials+science+%26+engineering&rft.au=Harris%2C+Thomas+I.&rft.au=Paterson%2C+Chase+A.&rft.au=Farjood%2C+Farhad&rft.au=Wadsworth%2C+Ian+D.&rft.date=2019-08-12&rft.issn=2373-9878&rft.eissn=2373-9878&rft.volume=5&rft.issue=8&rft.spage=4023&rft.epage=4036&rft_id=info:doi/10.1021%2Facsbiomaterials.9b00183&rft.externalDBID=n%2Fa&rft.externalDocID=10_1021_acsbiomaterials_9b00183
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2373-9878&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2373-9878&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2373-9878&client=summon