DNA Hole Transport on an Electrode:  Application to Effective Photoelectrochemical SNP Typing

• Published in: Journal of the American Chemical Society Vol. 128; no. 2; pp. 658 - 662
• Main Authors: Okamoto, Akimitsu, Kamei, Taku, Saito, Isao
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 18.01.2006
• Subjects: Base Sequence; Biological and medical sciences; DNA - chemistry; DNA Probes; Electrochemistry; Electrodes; Fundamental and applied biological sciences. Psychology; General pharmacology; Medical sciences; Models, Molecular; Molecular biophysics; Nucleic Acid Conformation; Oligonucleotides - chemistry; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions; Pharmacology. Drug treatments; Photochemistry; Physical chemistry in biology; Polymorphism, Single Nucleotide; Photoelectrochemical effect; Gold; Pharmacogenetics; Photosensitization; Solid electrode; Charge transport; Single nucleotide polymorphism; Oligodeoxyribonucleotide
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja057040t

A useful feature of DNA is that long-range hole transport through DNA is readily achieved. Photostimulated long-range hole transport through DNA has prospective use in the development of a conceptually new electrochemical single-nucleotide polymorphism (SNP) typing method for use as a versatile platform for gene diagnostics and pharmacogenetics. We have applied artificial DNAs designed for photostimulated long-range hole transport through DNA to SNP typing. By hybridizing photosensitizer-equipped DNA probes, immobilized on gold working electrodes, with a target DNA strand containing an SNP site, we observed a cathodic photocurrent, which markedly changed depending on the nature of the base at the specific site. The use of a combination of hole-transporting bases constitutes a very powerful method for a single-step electrochemical assay applicable to SNP typing of all types of sequences.