Determination of Collision Cross-Sections of Protein Ions in an Orbitrap Mass Analyzer

We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on the decay rate of the time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ions of ubiquitin, cytochrome c, and myoglobin were generated by electrospray ionization of both de...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 90; no. 9; pp. 5896 - 5902
Main Authors Sanders, James D, Grinfeld, Dmitry, Aizikov, Konstantin, Makarov, Alexander, Holden, Dustin D, Brodbelt, Jennifer S
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.05.2018
Subjects
Chemistry
Cross-sections
Cytochromes
Decay rate
Ionic mobility
Ionization
Ions
Mass spectrometry
Myoglobins
Proteins
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Summary:We demonstrate a method for determining the collision cross-sections (CCSs) of protein ions based on the decay rate of the time-domain transient signal from an Orbitrap mass analyzer. Multiply charged ions of ubiquitin, cytochrome c, and myoglobin were generated by electrospray ionization of both denaturing solutions and ones with high salt content to preserve native-like structures. A linear relationship between the pressure in the Orbitrap analyzer and the transient decay rate was established and used to demonstrate that the signal decay is primarily due to ion-neutral collisions for protein ions across the entire working pressure range of the instrument. The CCSs measured in this study were compared with previously published CCS values measured by ion mobility mass spectrometry (IMS), and results from the two methods were found to differ by less than 7% for all charge states known to adopt single gas-phase conformations.
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content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b00724