Therapeutic Potential of PTB Inhibition Through Converting Glial Cells to Neurons in the Brain

• Published in: Annual review of neuroscience Vol. 46; no. 1; pp. 145 - 165
• Main Authors: Fu, Xiang-Dong, Mobley, William C
• Format: Journal Article
• Language: English
• Published: United States Annual Reviews 10.07.2023; Annual Reviews, Inc
• Subjects: Astrocytes; Astrocytes - physiology; behavioral benefits; Brain; Glial cells; Humans; lineage tracing; Movement disorders; neurodegeneration; Neurodegenerative diseases; Neurogenesis; Neuroglia; neuronal reprogramming; Neuronal-glial interactions; Neurons; Neurons - physiology; Parkinson Disease; Parkinson's disease; pluripotent progenitors; Ptbp1; RNA-binding protein; neurodegeneration; Ptbp1; lineage tracing; behavioral benefits; pluripotent progenitors; neuronal reprogramming
• ISSN: 0147-006X; 1545-4126
• DOI: 10.1146/annurev-neuro-083022-113120

Cell replacement therapy represents a promising approach for treating neurodegenerative diseases. Contrary to the common addition strategy to generate new neurons from glia by overexpressing a lineage-specific transcription factor(s), a recent study introduced a subtraction strategy by depleting a single RNA-binding protein, Ptbp1, to convert astroglia to neurons not only in vitro but also in the brain. Given its simplicity, multiple groups have attempted to validate and extend this attractive approach but have met with difficulty in lineage tracing newly induced neurons from mature astrocytes, raising the possibility of neuronal leakage as an alternative explanation for apparent astrocyte-to-neuron conversion. This review focuses on the debate over this critical issue. Importantly, multiple lines of evidence suggest that Ptbp1 depletion can convert a selective subpopulation of glial cells into neurons and, via this and other mechanisms, reverse deficits in a Parkinson's disease model, emphasizing the importance of future efforts in exploring this therapeutic strategy.