Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures

The isomeric complexity of glycans make their analysis by traditional techniques particularly challenging. While the recent combination of ion mobility spectrometry (IMS) with cryogenic IR spectroscopy has demonstrated promise as a new technique for glycan analysis, this approach has been limited by...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 91; no. 7; pp. 4876 - 4882
Main Authors Ben Faleh, Ahmed, Warnke, Stephan, Rizzo, Thomas R
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 02.04.2019
Subjects
Analytical chemistry
Chemistry
Glycan
Infrared analysis
Infrared spectroscopy
Ionic mobility
Ions
Isomers
Mobility
Polysaccharides
Scientific imaging
Spectral analysis
Spectrometry
Spectroscopic analysis
Spectroscopy
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Summary:The isomeric complexity of glycans make their analysis by traditional techniques particularly challenging. While the recent combination of ion mobility spectrometry (IMS) with cryogenic IR spectroscopy has demonstrated promise as a new technique for glycan analysis, this approach has been limited by the modest resolution of the ion mobility stage. In this work we report results from a newly developed instrument that combines ultrahigh-resolution IMS with cryogenic IR spectroscopy for glycan analysis. This apparatus makes use of the recent development in traveling-wave IMS called structures for lossless ion manipulation. The IMS stage allows the selection of glycan isomers that differ in collisional cross section by as little as 0.2% before injecting them into a cryogenic ion trap for IR spectral analysis. We compare our results to those using drift-tube IMS and highlight the advantages of the substantial increase in resolution. Application of this approach to glycan mixtures demonstrates our ability to isolate individual components, measure a cryogenic IR spectrum, and identify them using a spectroscopic database.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b00659