Quantitative assessment of multiple pathogen exposure and immune dynamics at scale

Serology reveals exposure to pathogens, as well as the state of autoimmune and other clinical conditions. It is used to evaluate individuals and their histories and as a public health tool to track epidemics. Employing a variety of formats, studies nearly always perform serology by testing response...

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Published inMicrobiology spectrum Vol. 12; no. 1; p. e0239923
Main Authors Song, Lusheng, Rauf, Femina, Hou, Ching-Wen, Qiu, Ji, Murugan, Vel, Chung, Yunro, Lai, Huafang, Adam, Deborah, Magee, D Mitchell, Trivino Soto, Guillermo, Peterson, Milene, Anderson, Karen S, Rice, Stephen G, Readhead, Benjamin, Park, Jin G, LaBaer, Joshua
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 11.01.2024
Subjects
human microbiome
Humans
Immune System
immunoassays
Immunology
infectious disease surveillance
molecular diagnosis
multiplex assay
Public Health
Research Article
Serology - methods
virology
infectious disease surveillance
human microbiome
proteomics
virology
multiplex assay
immunoserology
immunoassays
molecular diagnosis
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Summary:Serology reveals exposure to pathogens, as well as the state of autoimmune and other clinical conditions. It is used to evaluate individuals and their histories and as a public health tool to track epidemics. Employing a variety of formats, studies nearly always perform serology by testing response to only one or a few antigens. However, clinical outcomes of new infections also depend on which previous infections may have occurred. We developed a high-throughput serology method that evaluates responses to hundreds of antigens simultaneously. It can be used to evaluate thousands of samples at a time and provide a quantitative readout. This tool will enable doctors to monitor which pathogens an individual has been exposed to and how that changes in the future. Moreover, public health officials could track populations and look for infectious trends among large populations. Testing many potential antigens at a time may also aid in vaccine development.
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Lusheng Song and Femina Rauf contributed equally to this article. Author order was determined in order of experiment finalization and manuscript editing.
The authors declare no conflict of interest.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.02399-23