Alcohol Pretreatment to Eliminate the Interference of Micro Additive Particles in the Identification of Microplastics Using Raman Spectroscopy

• Published in: Environmental science & technology Vol. 56; no. 17; pp. 12158 - 12168
• Main Authors: Li, Dunzhu, Sheerin, Emmet D., Shi, Yunhong, Xiao, Liwen, Yang, Luming, Boland, John J., Wang, Jing Jing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.09.2022
• Subjects: Additives; Contaminants in Aquatic and Terrestrial Environments; Environmental Monitoring - methods; Health risks; Interference; Microplastics; Plastic pollution; Plastics - chemistry; Polyethylene; Polyethylene - chemistry; Polyethylenes; Polypropylene; Polypropylenes - analysis; Polypropylenes - chemistry; Raman spectra; Raman spectroscopy; Spectroscopy; Spectrum analysis; Spectrum Analysis, Raman; Water Pollutants, Chemical - chemistry; alcohol pretreatment; microplastic; microadditive particles; hit quality index; Raman spectroscopy
• ISSN: 0013-936X; 1520-5851; 1520-5851
• DOI: 10.1021/acs.est.2c01551

Raman spectroscopy is an indispensable tool in the analysis of microplastics smaller than 20 μm. However, due to its limitation, Raman spectroscopy may be incapable of effectively distinguishing microplastics from micro additive particles. To validate this hypothesis, we characterized and compared the Raman spectra of six typical slip additives with polyethylene and found that their hit quality index values (0.93–0.96) are much higher than the accepted threshold value (0.70) used to identify microplastics. To prevent this interference, a new protocol involving an alcohol treatment step was introduced to successfully eliminate additive particles and accurately identify microplastics. Tests using the new protocol showed that three typical plastic products (polyethylene pellets, polyethylene bottle caps, and polypropylene food containers) can simultaneously release microplastic-like additive particles and microplastics regardless of the plastic type, daily-use scenario, or service duration. Micro additive particles can also adsorb onto and modify the surfaces of microplastics in a manner that may potentially increase their health risks. This study not only reveals the hidden problem associated with the substantial interference of additive particles in microplastic detection but also provides a cost-effective method to eliminate this interference and a rigorous basis to quantify the risks associated with microplastic exposure.