Direct Creation of Biopatterns via a Combination of Laser-Based Techniques and Click Chemistry

• Published in: Langmuir Vol. 33; no. 4; pp. 848 - 853
• Main Authors: Chatzipetrou, Marianneza, Massaouti, Maria, Tsekenis, George, Trilling, Anke K, van Andel, Esther, Scheres, Luc, Smulders, Maarten M. J, Zuilhof, Han, Zergioti, Ioanna
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 31.01.2017
• Subjects: Laboratorium voor Organische chemie; Laboratory for Organic Chemistry; Organic Chemistry; Organische Chemie; VLAG
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/acs.langmuir.6b02860

In this paper, we present the immobilization of thiol-modified aptamers on alkyne- or alkene-terminated silicon nitride surfaces by laser-induced click chemistry reactions. The aptamers are printed onto the surface by laser-induced forward transfer (LIFT), which also induces the covalent bonding of the aptamers by thiol–ene or thiol–yne reactions that occur upon UV irradiation of the thiol-modified aptamers with ns laser pulses. This combination of LIFT and laser-induced click chemistry allows the creation of high-resolution patterns without the need for masks. Whereas the click chemistry already takes place during the printing process (single-step process) by the laser pulse used for the printing process, further irradiation of the LIFT-printed aptamers by laser pulses (two-step process) leads to a further increase in the immobilization efficiency.