Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR Identifiler PCR Amplification Kit

Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, s...

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Published inJournal of forensic sciences Vol. 49; no. 6; p. 1265
Main Authors Collins, Patrick J, Hennessy, Lori K, Leibelt, Craig S, Roby, Rhonda K, Reeder, Dennis J, Foxall, Paul A
Format Journal Article
LanguageEnglish
Published United States 01.11.2004
Subjects
Animals
Bacteria - genetics
Cats
Cattle
Chickens
Deoxyribonucleotides
DNA Fingerprinting - methods
DNA Primers
Dogs
Genotype
Horses
Humans
Indicators and Reagents
Magnesium Chloride
Mice
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Potassium Chloride
Sequence Analysis, DNA
Species Specificity
Swine
Tandem Repeat Sequences
Temperature
Yeasts - genetics
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Summary:Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFlSTR kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFlSTR Identifiler PCR Amplification Kit for human identity and parentage testing.
ISSN:0022-1198
DOI:10.1520/JFS2002195