Bottom-up Layer-by-Layer Assembling of Antibacterial Freestanding Nanobiocomposite Films

• Published in: Biomacromolecules Vol. 19; no. 9; pp. 3628 - 3636
• Main Authors: Francesko, Antonio, Ivanova, Kristina, Hoyo, Javier, Pérez-Rafael, Sílvia, Petkova, Petya, Fernandes, Margarida M, Heinze, Thomas, Mendoza, Ernest, Tzanov, Tzanko
• Format: Journal Article Publication
• Language: English
• Published: United States American Chemical Society 10.09.2018
• Subjects: aminocellulose; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Biofilms - drug effects; biopolyme r-capped silver 1 nanoparticles; Biopolymers; Biopolímers; Cellulose - analogs & derivatives; Chitosan; Chitosan - analogs & derivatives; Enginyeria química; Escherichia coli - drug effects; freestanding antimicrobial films; hyaluronic acid; Hyaluronic Acid - chemistry; layer-by-layer; Metal Nanoparticles - chemistry; Nanocomposites - chemistry; Nanoparticles; Nanopartícules; Quitosan; Silver - chemistry; Staphylococcus aureus - drug effects; therapeutic use; Àrees temàtiques de la UPC; Ús terapèutic
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/acs.biomac.8b00626

In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA). At first, dispersions of AgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound. These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element. SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers. The composites of ≥50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli. Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs. Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds.