How Do Trehalose, Maltose, and Sucrose Influence Some Structural and Dynamical Properties of Lysozyme? Insight from Molecular Dynamics Simulations

• Published in: The journal of physical chemistry. B Vol. 111; no. 31; pp. 9410 - 9420
• Main Authors: Lerbret, A, Bordat, P, Affouard, F, Hédoux, A, Guinet, Y, Descamps, M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 09.08.2007
• Subjects: Animals; Chemical Sciences; Chickens; Computer Simulation; Maltose - metabolism; Maltose - pharmacology; Models, Molecular; Muramidase - chemistry; Muramidase - metabolism; Probability; Protein Conformation - drug effects; Solvents; Sucrose - metabolism; Sucrose - pharmacology; Trehalose - metabolism; Trehalose - pharmacology; Water - chemistry
• ISSN: 1520-6106; 1520-5207
• DOI: 10.1021/jp071946z

The influence of three well-known disaccharides, namely, trehalose, maltose, and sucrose, on some structural and dynamical properties of lysozyme has been investigated by means of molecular dynamics computer simulations in the 37−60 wt % concentration range. The effects of sugars on the protein conformation are found to be relatively weak, in agreement with the preferential hydration of lysozyme. Conversely, sugars seem to increase significantly the relaxation times of the protein. These effects are shown to be correlated to the fractional solvent accessibilities of lysozyme residues and further support the slaving of protein dynamics. Moreover, a significant increase in the relaxation times of lysozyme, sugars, and water molecules is observed within the studied concentration range and may result from the percolation of the hydrogen-bond network of sugar molecules. This percolation appears to be of primary importance to explain the influence of sugars on the dynamical properties of lysozyme and water.