Binding of Daptomycin to Anionic Lipid Vesicles Is Reduced in the Presence of Lysyl-Phosphatidylglycerol

The cytoplasmic membrane of Staphylococcus aureus contains ∼20 mol% of the net cationic lipid lysyl-phosphatidylglycerol (LPG). Elevated fractions of LPG are associated with increased resistance to cationic antibiotics, including the lipopeptide daptomycin (DAP). Although the surface charge of the b...

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Published inAntimicrobial agents and chemotherapy Vol. 60; no. 8; pp. 5051 - 5053
Main Authors Khatib, Tala O, Stevenson, Heather, Yeaman, Michael R, Bayer, Arnold S, Pokorny, Antje
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.08.2016
Subjects
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Cell Membrane - drug effects
Cell Membrane - metabolism
Daptomycin
Daptomycin - chemistry
Daptomycin - pharmacology
Lipids
Lipids - chemistry
Lysine
Lysine - chemistry
Mechanisms of Resistance
Microbial Sensitivity Tests
Phosphatidylglycerols
Phosphatidylglycerols - chemistry
Staphylococcus aureus
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Summary:The cytoplasmic membrane of Staphylococcus aureus contains ∼20 mol% of the net cationic lipid lysyl-phosphatidylglycerol (LPG). Elevated fractions of LPG are associated with increased resistance to cationic antibiotics, including the lipopeptide daptomycin (DAP). Although the surface charge of the bacterial cytoplasmic membrane is altered by LPG, surface binding of DAP was found to be only moderately affected in anionic vesicles containing 20 mol% LPG. These results suggest that charge repulsion cannot fully explain LPG-mediated resistance to cationic peptides.
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Citation Khatib TO, Stevenson H, Yeaman MR, Bayer AS, Pokorny A. 2016. Binding of daptomycin to anionic lipid vesicles is reduced in the presence of lysyl-phosphatidylglycerol. Antimicrob Agents Chemother 60:5051–5053. doi:10.1128/AAC.00744-16.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00744-16