Analytical and functional determination of polymyxin B protein binding in serum

To enhance our understanding of the pharmacological properties of polymyxin B, serum protein binding for polymyxin B1, B2, and B3 and for isoleucine-polymyxin B1 was evaluated. Using equilibrium dialysis and ultrafiltration, comparable protein binding was found in all 4 components of polymyxin B (92...

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Published inAntimicrobial agents and chemotherapy Vol. 59; no. 11; pp. 7121 - 7123
Main Authors Abodakpi, Henrietta, Gohlke, Jie, Chang, Kai-Tai, Chow, Diana S-L, Tam, Vincent H
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.11.2015
Subjects
Humans
Pharmacology
Polymyxin B
Polymyxin B - blood
Polymyxin B - metabolism
Polymyxins - analogs & derivatives
Polymyxins - blood
Polymyxins - metabolism
Protein Binding
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Summary:To enhance our understanding of the pharmacological properties of polymyxin B, serum protein binding for polymyxin B1, B2, and B3 and for isoleucine-polymyxin B1 was evaluated. Using equilibrium dialysis and ultrafiltration, comparable protein binding was found in all 4 components of polymyxin B (92% to 99%). Protein binding in human serum was further assessed using a functional assay, the results of which were in general agreement with previous findings (approximately 90%).
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Citation Abodakpi H, Gohlke J, Chang K-T, Chow DS-L, Tam VH. 2015. Analytical and functional determination of polymyxin B protein binding in serum. Antimicrob Agents Chemother 59:7121–7123. doi:10.1128/AAC.01815-15.
Present address: Jie Gohlke, Baylor College of Medicine, Houston, Texas, USA.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01815-15