Differentiation of citrus juices by factorial discriminant analysis using liquid chromatography of flavanone glycosides

• Published in: Journal of agricultural and food chemistry Vol. 42; no. 1; pp. 70 - 79
• Main Authors: Mouly, Pierre P, Arzouyan, Claude R, Gaydou, Emile M, Estienne, Jacques M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.01.1994
• Subjects: alimentos; Biological and medical sciences; chromatographie; chromatography; citron; Citrus; cromatografia; flavonoide; flavonoides; flavonoids; Food industries; foods; Fruit and vegetable industries; fruit juices; Fundamental and applied biological sciences. Psychology; grapefruits; jugo de frutas; jus d' orange; jus de fruit; lemons; lima; lime; limes; limon acido; orange juice; pomelo; produit alimentaire; toronja; zumo de naranja; Statistical analysis; Citrus fruit; HPLC chromatography; Glycoside; Identification; Flavonoid; Fruit juice
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00037a011

Flavanone glycosides (FG) found in citrus juices have been separated and quantitatively determined by reversed-phase liquid chromatography (LC) using a C18 packed column and water-acetonitrile-tetrahydrofuran-glacial acetic acid eluent system. The influence of juice pH on hesperidin and narirutin determinations, from commercial orange juices, has been studied at different pH values (from 2.0 to 6.2). The response factors, averages, relative standard deviations, and recoveries of six FG were determined using LC with an UV detector at 280 nm. The six FG and three unknown compounds found in citrus juices were determined in 124 samples of lemons, limes, grapefruits, and sweet oranges. The major FG in each citrus group were as follows: in lemon and lime, eriocitrin (47-94 mg L-1) and hesperidin (84-196 mg L-1); in sweet orange, narirutin (30-84 mg L-1) and hesperidin (235-407 mg L-1); in grapefruit, narirutin (33-161 mg L-1) and naringin (113-481 mg L-1). Factorial discriminant analysis of the data obtained effectively differentiated lemon and lime and varieties of grapefruits (white, pink, red, and green) and sweet oranges (Valencia, navel, blood, Thomson, and Malta).