Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads

This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization...

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Published inEnzyme Research Vol. 2014; no. 2014; pp. 202 - 208
Main Authors Bonine, Bárbara M., Polizelli, Patricia Peres, Bonilla-Rodriguez, Gustavo O.
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Limiteds 2014
Hindawi Puplishing Corporation
Hindawi Publishing Corporation
Hindawi Limited
Subjects
Aqueous solutions
Enzymes
Standard deviation
Temperature
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Summary:This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Academic Editor: Raffaele Porta
ISSN:2090-0414
2090-0406
2090-0414
DOI:10.1155/2014/738739