Development of monoclonal antibodies targeting the conserved fragment of hexon protein to detect different serotypes of human adenovirus

• Published in: Microbiology spectrum Vol. 12; no. 4; p. e0181623
• Main Authors: Wu, Linfan, Lin, Yuhao, Yin, Juzhen, Yang, Changbi, Jiang, Yushan, Zhai, Linlin, Wang, Yuelin, Zhu, Li, Wu, Qinghua, Zhang, Bao, Wan, Chengsong, Zhao, Wei, Yang, Yang, Shen, Chenguang, Xiao, Weiwei
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 02.04.2024
• Subjects: Adenovirus Infections, Human; Adenoviruses, Human - genetics; Animals; Antibodies, Monoclonal; Antibodies, Viral; Capsid Proteins - genetics; ELISA; hexon protein; human adenovirus; Humans; indirect immunofluorescence assay; Mice; monoclonal antibodies; Research Article; Serogroup; Virology; Western blot; Western blot; human adenovirus; hexon protein; indirect immunofluorescence assay; ELISA; monoclonal antibodies
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.01816-23

In this study, we selected the three most conserved antigenic fragments of human adenovirus to prepare a murine monoclonal antibody for the first time, and human adenovirus antigenic fragments with heretofore unheard of degrees of conservatism were isolated. The three monoclonal antibodies with the ability to recognize human respiratory adenovirus over a broad spectrum were screened by hybridoma and monoclonal antibody preparation. Human adenovirus infections are serious; however, therapeutic drugs and diagnostic reagents are scarce. Thus, to reduce the serious consequences of human viral infections and adenovirus pneumonitis, early diagnosis of infection is required. The present study provides three monoclonal antibodies capable of recognizing a wide range of human adenoviruses, thereby offering guidance for subsequent research and development.