An Improved, Rapid in Vitro Method To Measure Antioxidant Activity. Application on Selected Flavonoids and Apple Juice

• Published in: Journal of agricultural and food chemistry Vol. 48; no. 9; pp. 4116 - 4122
• Main Authors: van der Sluis, Addie A, Dekker, Matthijs, Verkerk, Ruud, Jongen, Wim M. F
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.09.2000
• Subjects: ANALYTICAL METHODS; Animals; ANTIOXIDANTES; ANTIOXIDANTS; Antioxidants - pharmacology; ANTIOXYDANT; APPLE JUICE; Beverages - analysis; Biological and medical sciences; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Flavonoids - pharmacology; Food industries; Fruit and vegetable industries; FRUIT JUICES; Fundamental and applied biological sciences. Psychology; IN VITRO; In Vitro Techniques; JUGO DE FRUTAS; JUS DE FRUITS; JUS DE POMME; Leerstoelgroep Productontwerpen en kwaliteitskunde; Male; Microsomes, Liver - drug effects; Microsomes, Liver - metabolism; Product Design and Quality Management Group; Rats; Rats, Wistar; Rosales - chemistry; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; VLAG; ZUMO DE MANZANA; Apple; Fruit; Mathematical method; Measurement method; Antioxidant; Microsome; Flavonoid; In vitro; Fruit juice; ELISA assay; Biological activity; Quantitative analysis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf000156i

A rapid in vitro method for measuring antioxidant activity is presented, which enables the evaluation of health claims and the optimization of product development with respect to health protecting compounds. Antioxidant activity is assessed in a system in which lipid peroxidation is induced in male rat liver microsomes by ascorbic acid and FeSO4. This method has been significantly improved by enabling the use of microtiter plates and an ELISA reader. Large numbers of samples can be analyzed with good reproducibility, which is necessary when dealing with microsomes possessing biological variability. An objective mathematical procedure has been developed to translate data obtained from the lipid peroxidation assay into a value describing the antioxidant activity. As an illustration the method has been applied to measure antioxidant activity of individual flavonoids and apple juice. Keywords: Antioxidant activity; flavonoids; assay; apple juice