Effect of Feeding Pomegranate Byproduct on Fatty Acid Composition of Ruminal Digesta, Liver, and Muscle in Lambs

• Published in: Journal of agricultural and food chemistry Vol. 67; no. 16; pp. 4472 - 4482
• Main Authors: Natalello, Antonio, Luciano, Giuseppe, Morbidini, Luciano, Valenti, Bernardo, Pauselli, Mariano, Frutos, Pilar, Biondi, Luisa, Rufino-Moya, Pablo J, Lanza, Massimiliano, Priolo, Alessandro
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.04.2019
• Subjects: Animal Feed - analysis; Animals; Fatty Acids - chemistry; Fatty Acids - metabolism; Liver - chemistry; Liver - metabolism; Meat - analysis; Muscle, Skeletal - chemistry; Muscle, Skeletal - metabolism; Punicaceae - chemistry; Punicaceae - metabolism; Rumen - chemistry; Rumen - metabolism; Sheep - growth & development; Sheep - metabolism; Waste Products - analysis; meat quality; liver; conjugated linoleic and linolenic acids; fatty acid metabolism; rumen; pomegranate
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.9b00307

This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with a concentrate diet containing 200 g/kg DM of WPB to partially replace barley and corn (WPB). The dietary treatment did not affect the final body and carcass weight, the dry matter intake, or the average daily gain. However, total polyunsaturated FA (PUFA), linolenic, rumenic (RA), and vaccenic (VA) acid were increased in liver (+15%, +32%, +344%, and +118%, respectively) and muscle (+46%, +38%, +169%, and +89%, respectively) of WPB lambs (P < 0.05). Punicic acid and three isomers of conjugated linolenic acid were detected exclusively in the rumen and tissues of WPB-lambs. The C18:1 t10/t11 ratio in rumen digesta or in tissues was reduced by feeding WPB (−791%, −690%, and −456%, respectively, in rumen, liver and muscle; P < 0.001), suggesting that the WPB prevented the t10-shift rumen biohydrogenation pathway. In conclusion, the inclusion of WPB into a concentrate-based diet can be a strategy to improve the FA composition of meat, without effects on the animal performances.