Selected cachaça yeast strains share a genomic profile related to traits relevant to industrial fermentation processes

• Published in: Applied and environmental microbiology Vol. 90; no. 1; p. e0175923
• Main Authors: Campos, Anna Clara Silva, Araújo, Thalita Macedo, Moraes, Lauro, Corrêa dos Santos, Renato Augusto, Goldman, Gustavo Henrique, Riano-Pachon, Diego Maurício, Oliveira, Juliana Velasco de Castro, Squina, Fabio Marcio, Castro, Ieso de Miranda, Trópia, Maria José Magalhães, da Cunha, Aureliano Claret, Rosse, Izinara C., Brandão, Rogelio Lopes
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 24.01.2024
• Subjects: Acetic acid; Alcohols; Annotations; Applied and Industrial Microbiology; Beverages; Biotechnology; Drug tolerance; Esters; Ethanol; Ethyl esters; Fermentation; Flocculation; Gene sequencing; Genes; Genetic diversity; Genetic Profile; Genome editing; Genomes; Genomics; Literature reviews; Microorganisms; Network analysis; Nucleotides; Osmolarity; Phenotype; Phenotypes; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Strains (organisms); Strategy; Sugarcane; Volatile compounds; Whole genome sequencing; Yeast; Saccharomyces cerevisiae; SNVs; comparative genomics; biotechnology for the fermentation industry
• ISSN: 0099-2240; 1098-5336; 1098-5336
• DOI: 10.1128/aem.01759-23

The isolation and selection of yeast strains to improve the quality of the cachaça —Brazilian Spirit—have been studied in our research group. Our strategy considers Saccharomyces cerevisiae as the predominant species involved in sugarcane juice fermentation and the presence of different stressors (osmolarity, temperature, ethanol content, and competition with other microorganisms). It also considers producing balanced concentrations of volatile compounds (higher alcohols and acetate and/or ethyl esters), flocculation capacity, and ethanol production. Since the genetic bases behind these traits of interest are not fully established, the whole genome sequencing of 11 different Saccharomyces cerevisiae strains isolated and selected from different places was analyzed to identify the presence of a specific genetic variation common to cachaça yeast strains. We have identified 20,128 single-nucleotide variants shared by all genomes. Of these shared variants, 37 were new variants (being six missenses), and 4,451 were identified as missenses. We performed a detailed functional annotation (using enrichment analysis, protein–protein interaction network analysis, and database and in-depth literature searches) of these new and missense variants. Many genes carrying these variations were involved in the phenotypes of flocculation, tolerance to fermentative stresses, and production of volatile compounds and ethanol. These results demonstrate the existence of a genetic profile shared by the 11 strains under study that could be associated with the applied selective strategy. Thus, this study points out genes and variants that may be used as molecular markers for selecting strains well suited to the fermentation process, including genetic improvement by genome editing, ultimately producing high-quality beverages and adding value. This work demonstrates the existence of new genetic markers related to different phenotypes used to select yeast strains and mutations in genes directly involved in producing flavoring compounds and ethanol, and others related to flocculation and stress resistance.