Magnesium Ion Selective Two-Photon Fluorescent Probe Based on a Benzo[h]chromene Derivative for in Vivo Imaging

• Published in: Journal of organic chemistry Vol. 72; no. 6; pp. 2088 - 2096
• Main Authors: Kim, Hwan Myung, Yang, Pil Rye, Seo, Mun Sik, Yi, Jae-Sung, Hong, Jin Hee, Jeon, Seung-Joon, Ko, Young-Gyu, Lee, Kyoung J, Cho, Bong Rae
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 16.03.2007; Amer Chemical Soc
• Subjects: Animals; Benzopyrans; Cells - chemistry; Chemistry; Chemistry, Organic; Exact sciences and technology; Fluorescent Dyes; Heterocyclic compounds; Heterocyclic compounds with o, s, se, te hetero atom and condensed derivatives; Humans; Magnesium - analysis; Magnesium - pharmacokinetics; Organic chemistry; Photons; Physical Sciences; Preparations and properties; Science & Technology; CROSS-SECTIONS; NMR; FLUOROPHORES; CALCIUM; SENSOR; MG2; METAL-IONS; MICROSCOPY; MODULATION; ABSORPTION PROPERTIES; In vivo; Calcium ion; Imaging; Laser; Magnesium ion; Fluorescence; Dissociation constant; Selectivity; Fluorescent compound; Detection; Oxygen heterocycle
• ISSN: 0022-3263; 1520-6904
• DOI: 10.1021/jo062341m

A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (K d i) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).