Metal Contamination Effects on Sunflower (Helianthus annuus L.) Growth and Protein Expression in Leaves During Development

• Published in: Journal of agricultural and food chemistry Vol. 54; no. 22; pp. 8623 - 8630
• Main Authors: Garcia, Jerusa Simone, Gratão, Priscila Lupino, Azevedo, Ricardo Antunes, Arruda, Marco Aurélio Zezzi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.11.2006
• Subjects: Biological and medical sciences; Biomass; Food industries; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Helianthus - drug effects; Helianthus - growth & development; Helianthus - metabolism; Ions - chemistry; Metals, Heavy - chemistry; Metals, Heavy - pharmacology; Molecular Weight; Plant Leaves - drug effects; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Reactive Oxygen Species - metabolism; Cadmium; metal stress; Growth; Plant leaf; Compositae; Inorganic element; Contamination; Protein; Stress; Zinc; Heavy metal; Helianthus annuus; Plant part; Dicotyledones; proteins; Angiospermae; Development; Lead; Spermatophyta; Copper; Sunflower; Sunflowers
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf061593l

Metal-ion contamination (Cd, Cu, Pb, and Zn) on sunflower (Helianthus annuus L.) growth and total leaf protein expression were studied in the present work. The height, mass production, and metal distribution (Ca, K, Fe, Mg, Na, and P) in all plant fractions (roots, stems, and leaves) were evaluated. Sunflowers plants contaminated with four metal ions decreases height and mass by 35% and 40%, respectively, compared to control. Significant differences of total protein composition were noted after SDS-PAGE separation. Sunflower proteomics were more affected when 500 mg L-1 of metal ion was added as contaminant of both zinc and mixed ions solution. In these cases, proteins having a molar mass of 14.5, 34.5, and 54.0 kDa were present at a lower level and alterations in enzymatic activities (SOD and GR) were found. Sunflowers plants contaminated with zinc and the mixed ions solution showed some degree of oxidative stress. Keywords: Sunflowers; proteins; metal stress; cadmium; copper; lead; zinc