MicroRNA Detection: Current Technology and Research Strategies

The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential i...

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Bibliographic Details
Published inAnnual review of analytical chemistry (Palo Alto, Calif.) Vol. 8; p. 217
Main Authors Hunt, Eric A, Broyles, David, Head, Trajen, Deo, Sapna K
Format Journal Article
LanguageEnglish
Published United States 22.07.2015
Subjects
Animals
High-Throughput Nucleotide Sequencing - methods
Humans
MicroRNAs - analysis
Real-Time Polymerase Chain Reaction - methods
Northern blot
microarray
biosensors
RNA-seq
ncRNA
RT-qPCR
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Summary:The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques [such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB)] and relatively recent advancements [such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNA/targets] to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.
ISSN:1936-1335
DOI:10.1146/annurev-anchem-071114-040343