Stereoselective Bioreduction of Bulky-Bulky Ketones by a Novel ADH from Ralstonia sp

• Published in: Journal of organic chemistry Vol. 73; no. 15; pp. 6003 - 6005
• Main Authors: Lavandera, Iván, Kern, Alexander, Ferreira-Silva, Bianca, Glieder, Anton, de Wildeman, Stefaan, Kroutil, Wolfgang
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.08.2008; Amer Chemical Soc
• Subjects: Alcohol Dehydrogenase - metabolism; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Catalysis; Chemistry; Chemistry, Organic; Enzymes and enzyme inhibitors; Escherichia coli - enzymology; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; Ketones - chemistry; Ketones - metabolism; Molecular Structure; Oxidation-Reduction; Physical Sciences; Ralstonia - enzymology; ras Proteins - metabolism; Science & Technology; Substrate Specificity; OXIDATION; SYSTEM; BROAD SUBSTRATE-SPECIFICITY; ALCOHOL-DEHYDROGENASE; ASYMMETRIC REDUCTION; ENANTIOSELECTIVE REDUCTION; CHIRAL ALCOHOLS; CARBONYL REDUCTASE; TOOL; NADPH; Biocatalysis; Enzyme; Escherichia coli; Ralstonia; Alcohol; Steric hindrance; Alcohol dehydrogenase; Ketone; Chemical reduction; Stereoselectivity; Bacteria; Oxidoreductases; Enterobacteriaceae
• ISSN: 0022-3263; 1520-6904
• DOI: 10.1021/jo800849d

Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.