A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement

Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Farada...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 127; no. 51; pp. 17990 - 17991
Main Authors Xiao, Yi, Piorek, Brian D, Plaxco, Kevin W, Heeger, Alan J
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 28.12.2005
Subjects
Aptamers, Nucleotide - chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
DNA - chemistry
Electrochemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Methods. Procedures. Technologies
Thrombin - chemistry
Various methods and equipments
Thiazine dye
Performance evaluation
Molecular hybridization
Biosensor
Oligonucleotide
Methylene blue
Electrochemical detector
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Summary:Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM.
Bibliography:ark:/67375/TPS-37PT3ZSZ-8
istex:75AF23DDEC03FDCAA122A2513F17D3796FD05060
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja056555h