Membrane-Binding Biomolecules Influence the Rate of Vesicle Exchange between Bacteria

• Published in: Applied and environmental microbiology Vol. 88; no. 23; p. e0134622
• Main Authors: Tran, Frances, Gangan, Manasi S, Weaver, Brian P, Boedicker, James Q
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 13.12.2022
• Subjects: Antiinfectives and antibacterials; Antimicrobial peptides; Bacteria; Bacteria - genetics; Bacteria - metabolism; Bacterial vesicles; Binding; Biomolecules; Colistin; Eukaryota; Evolutionary and Genomic Microbiology; Extracellular Vesicles - metabolism; Gene Transfer, Horizontal; Horizontal cells; Horizontal transfer; Membrane structure; Membrane structures; Membranes; Microbial Genetics; Molecular structure; Peptides; Polymyxin B; Vesicles; horizontal gene transfer; membrane biophysics; vesicles
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.01346-22

The exchange of bacterial extracellular vesicles facilitates molecular exchange between cells, including the horizontal transfer of genetic material. Given the implications of such transfer events on cell physiology and adaptation, some bacterial cells have likely evolved mechanisms to regulate vesicle exchange. Past work has identified mechanisms that influence the formation of extracellular vesicles, including the production of small molecules that modulate membrane structure; however, whether these mechanisms also modulate vesicle uptake and have an overall impact on the rate of vesicle exchange is unknown. Here, we show that membrane-binding molecules produced by microbes influence both the formation and uptake of extracellular vesicles and have the overall impact of increasing the vesicle exchange rate within a bacterial coculture. In effect, production of compounds that increase vesicle exchange rates encourage gene exchange between neighboring cells. The ability of several membrane-binding compounds to increase vesicle exchange was demonstrated. Three of these compounds, nisin, colistin, and polymyxin B, are antimicrobial peptides added at sub-inhibitory concentrations. These results suggest that a potential function of exogenous compounds that bind to membranes may be the regulation of vesicle exchange between cells. The exchange of bacterial extracellular vesicles is one route of gene transfer between bacteria, although it was unclear if bacteria developed strategies to modulate the rate of gene transfer within vesicles. In eukaryotes, there are many examples of specialized molecules that have evolved to facilitate the production, loading, and uptake of vesicles. Recent work with bacteria has shown that some small molecules influence membrane curvature and induce vesicle formation. Here, we show that similar compounds facilitate vesicle uptake, thereby increasing the overall rate of vesicle exchange within bacterial populations. The addition of membrane-binding compounds, several of them antibiotics at subinhibitory concentrations, to a bacterial coculture increased the rate of horizontal gene transfer via vesicle exchange.