Chip-Based High-Performance Liquid Chromatography for High-Speed Enantioseparations

• Published in: Analytical chemistry (Washington) Vol. 87; no. 11; pp. 5568 - 5576
• Main Authors: Thurmann, Sebastian, Lotter, Carsten, Heiland, Josef J, Chankvetadze, Bezhan, Belder, Detlev
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.06.2015
• Subjects: Analytical chemistry; Cellulose; Cellulose - analogs & derivatives; Cellulose - chemistry; Chemistry Techniques, Analytical - methods; Chips; Chromatography; Chromatography, High Pressure Liquid; Enantiomers; Fluorescence; Glass; Liquid chromatography; Mass transfer; Microchip Analytical Procedures; Microfluidics; Phase transitions; Phenylcarbamates - chemistry; Separation; Silicon Dioxide - chemistry; Stereoisomerism; Time Factors
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.5b00210

In this work, the first high-performance chiral liquid chromatography in packed microfluidic chips is presented. The chromatographic separation was performed on a column integrated into the microfluidic glass chip and packed with the particulate chiral stationary phase. Cellulose tris­(3,5-dimethylphenylcarbamate) coated on 5-μm fully porous silica was used as chiral stationary phase material. Several racemic analytes including pharmaceutical products were baseline separated into their corresponding enantiomers under reversed-phase, polar organic and normal-phase conditions, demonstrating the versatility of the glass chip in the field of chiral separations. Van Deemter plots revealed a reduced plate height of 2.2 and a trend to enhanced mass transfer processes for solutes under low retention conditions. The utilization of very short column lengths of down to 12 mm led to ultrafast separations of enantiomers within 5 s.