Production of l -Theanine by Escherichia coli in the Absence of Supplemental Ethylamine

l -Theanine is widely used in food additives and dietary supplements. Industrial production of l -theanine uses the toxic and highly flammable precursor ethylamine, raising production costs. In this study, we used Escherichia coli to engineer two biosynthetic pathways that produce l -theanine from g...

Full description

Saved in:
Bibliographic Details
Published inApplied and environmental microbiology Vol. 87; no. 11; pp. 1 - 12
Main Authors Hagihara, Ryota, Ohno, Shoto, Hayashi, Mikiro, Tabata, Kazuhiko, Endo, Hirofumi
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 11.05.2021
Subjects
Acetaldehyde
Alanine
Amino acids
Biotechnology
Condensates
E coli
Escherichia coli
Flammability
Glutamic acid
Glutamine
Industrial production
L-Alanine
Production methods
Proteins
Pseudomonas putida
Theanine
Transaminase
γ-Glutamyltransferase
alanine decarboxylase
l-theanine
γ-glutamylmethylamide synthetase
Escherichia coli
ω-transaminase
theanine hydrolase
Online AccessGet full text

Cover

More Information
Summary:l -Theanine is widely used in food additives and dietary supplements. Industrial production of l -theanine uses the toxic and highly flammable precursor ethylamine, raising production costs. In this study, we used Escherichia coli to engineer two biosynthetic pathways that produce l -theanine from glucose and ammonia in the absence of supplemental ethylamine. This study establishes a foundation for safely and economically producing l -theanine.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Citation Hagihara R, Ohno S, Hayashi M, Tabata K, Endo H. 2021. Production of l-theanine by Escherichia coli in the absence of supplemental ethylamine. Appl Environ Microbiol 87:e00031-21. https://doi.org/10.1128/AEM.00031-21.
ISSN:0099-2240
1098-5336
1098-5336
DOI:10.1128/AEM.00031-21