Role of the Conserved DECH-Box Cysteine in Coupling Hepatitis C Virus Helicase-Catalyzed ATP Hydrolysis to RNA Unwinding

• Published in: Biochemistry (Easton) Vol. 57; no. 43; pp. 6247 - 6255
• Main Authors: Yerukhimovich, Mark M, Marohnic, Christopher C, Frick, David N
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 30.10.2018
• Subjects: Adenosine Triphosphate - metabolism; Binding Sites; Catalysis; Cysteine - chemistry; Cysteine - genetics; Cysteine - metabolism; DNA - chemistry; DNA - metabolism; Hepacivirus - enzymology; Humans; Hydrolysis; Mutagenesis, Site-Directed; Mutation; RNA - chemistry; RNA - metabolism; Substrate Specificity; Viral Nonstructural Proteins - chemistry; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/acs.biochem.8b00796

DECH-box proteins are a subset of DExH/D-box superfamily 2 helicases possessing a conserved Asp-Glu-Cys-His motif in their ATP binding site. The conserved His helps position the Asp and Glu residues, which coordinate the divalent metal cation that connects the protein to ATP and activate the water molecule needed for ATP hydrolysis, but the role of the Cys is still unclear. This study uses site-directed mutants of the model DECH-box helicase encoded by the hepatitis C virus (HCV) to examine the role of the Cys in helicase action. Proteins lacking a Cys unwound DNA less efficiently than wild-type proteins did. For example, at low protein concentrations, a helicase harboring a Gly instead of the DECH-box Cys unwound DNA more slowly than the wild-type helicase did, but at higher protein concentrations, the two proteins unwound DNA at similar rates. All HCV proteins analyzed had similar affinities for ATP and nucleic acids and hydrolyzed ATP in the presence of RNA at similar rates. However, in the absence of RNA, all proteins lacking a DECH-box cysteine hydrolyzed ATP 10–15 times faster with higher K m values, and lower apparent affinities for metal ions, compared to those observed with wild-type proteins. These differences were observed with proteins isolated from HCV genotypes 2a and 1b, suggesting that this role is conserved. These data suggest the helicase needs Cys292 to bind ATP in a state where ATP is not hydrolyzed until RNA binds.