Aptamer-Based Colorimetric Probe for Cocaine
Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of...
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| Published in | Journal of the American Chemical Society Vol. 124; no. 33; pp. 9678 - 9679 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Chemical Society
21.08.2002
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be followed with the unaided eye as displaced dye precipitates and supernatant decolorizes. |
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| AbstractList | Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2-600 muM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be followed with the unaided eye as displaced dye precipitates and supernatant decolorizes. Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be followed with the unaided eye as displaced dye precipitates and supernatant decolorizes. |
| Author | Landry, Donald W Stojanovic, Milan N |
| Author_xml | – sequence: 1 givenname: Milan N surname: Stojanovic fullname: Stojanovic, Milan N – sequence: 2 givenname: Donald W surname: Landry fullname: Landry, Donald W |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13845620$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/12175205$$D View this record in MEDLINE/PubMed |
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| Snippet | Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine... |
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| SubjectTerms | Analytical chemistry Base Sequence Carbocyanines - chemistry Chemistry Cocaine - analysis Cocaine - chemistry Colorimetry - methods Coloring Agents - chemistry Exact sciences and technology Miscellaneous Molecular Sequence Data Oligonucleotides - chemistry Spectrophotometry - methods |
| Title | Aptamer-Based Colorimetric Probe for Cocaine |
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