Aptamer-Based Colorimetric Probe for Cocaine

Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 124; no. 33; pp. 9678 - 9679
Main Authors Stojanovic, Milan N, Landry, Donald W
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 21.08.2002
Subjects
Analytical chemistry
Base Sequence
Carbocyanines - chemistry
Chemistry
Cocaine - analysis
Cocaine - chemistry
Colorimetry - methods
Coloring Agents - chemistry
Exact sciences and technology
Miscellaneous
Molecular Sequence Data
Oligonucleotides - chemistry
Spectrophotometry - methods
Chemistry
Cocaine
Colorimetry
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Summary:Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be followed with the unaided eye as displaced dye precipitates and supernatant decolorizes.
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ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja0259483