Comparison of Spectroscopic Techniques for the Determination of Kjeldahl and Ammoniacal Nitrogen Content of Farmyard Manure

• Published in: Journal of agricultural and food chemistry Vol. 49; no. 2; pp. 603 - 609
• Main Authors: Kemsley, E. Katherine, Tapp, Henri S, Scarlett, Andrew J, Miles, Simon J, Hammond, Robert, Wilson, Reginald H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.02.2001
• Subjects: Agronomy. Soil science and plant productions; Animals; Biological and medical sciences; Cattle; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Indicators and Reagents; Manure - analysis; Nitrogen - analysis; Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries; Regression Analysis; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Spectrophotometry, Infrared - methods; Spectroscopy, Fourier Transform Infrared - methods; Swine; Turkeys; Near infrared spectrometry; Ammonium; Kjeldahl method; Nitrates; Concentration; Measurement method; Photoacoustic spectrometry; Nitrogen; Farmyard manure; Optothermal spectrometry; Comparative study; Reflectance
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf001060r

The feasibility of determining the nitrogen content of farmyard manure using infrared spectroscopy was investigated. Fifteen samples each of cattle, pig, and turkey manure were analyzed by three infrared techniques:  Fourier transform mid-infrared (MIR), using attenuated total reflection (ATR); near-infrared reflectance (NIR-R); and near-infrared optothermal photoacoustic (NIR-OT). The near-infrared measurements were made at wavelengths determined respectively by four (NIR-OT) and five (NIR-R) band-pass filters. The total nitrogen (using the Kjeldahl method) and volatile (ammoniacal) nitrogen contents of all samples were measured by wet chemistry. Internally cross-validated (ICV) partial least-squares (PLS) regression was then used to obtain calibrations for the nitrogen content. The data sets obtained by each technique were treated separately. Within these sets, data from each manure type were treated both separately and combined:  the best predictive ability was obtained by combining data from all three manure types. From the combined data set, the residual standard deviations and correlation coefficients for the ICV-predicted versus actual Kjeldahl nitrogen content were, respectively, 6772 mg/kg dry wt, 0.862 (MIR); 9434 mg/kg dry wt, 0.771 (NIR-OT); and 8943 mg/kg dry wt, 0.865 (NIR-R). For the ammoniacal nitrogen content, the residual standard deviations and correlation coefficients were 3869 mg/kg dry wt, 0.899 (MIR); 6079 mg/kg dry wt, 0.820 (NIR-OT); and 3498 mg/kg dry wt, 0.961 (NIR-R). Keywords: Nitrogen; manure; near-infrared; mid-infrared; optothermal; spectroscopy