Effect of High-Pressure Treatment on the Texture of Cherry Tomato

• Published in: Journal of agricultural and food chemistry Vol. 48; no. 5; pp. 1434 - 1441
• Main Authors: Tangwongchai, Ratchada, Ledward, Dave A, Ames, Jennifer M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2000
• Subjects: Biological and medical sciences; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Lycopersicon esculentum - cytology; Lycopersicon esculentum - enzymology; Plant Viral Movement Proteins; Polygalacturonase - metabolism; Pressure; Viral Proteins - metabolism; Physical dressing; Enzyme; Esterases; Polygalacturonase; Softening; Tomato; Texture; Carboxylic ester hydrolases; High pressure; Enzymatic activity; Glycosidases; Quality; Hydrolases; O-Glycosidases; Vegetable; Pectinesterase
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf990796p

The effect of high-pressure treatment (200−600 MPa for 20 min) on the texture of cherry tomatoes and on the key softening enzymes (pectinmethylesterase and polygalacturonase) was investigated. When subjected to high-pressure treatment whole cherry tomatoes showed increasing textural damage with increasing pressures up to 400 MPa. However, treatment at pressures above 400 MPa (500−600 MPa) led to less apparent damage than treatment at 300 and 400 MPa; the tomatoes appearing more like the untreated samples. These visual changes were reflected in the texture (firmness) and amount of cell rupture in the tomatoes, with the least firmness and the most cell rupture being seen after treatment at 400 MPa. Light and scanning electron microscopy supported these observations. Although a sample of purified commercial pectinmethylesterase was partially inactivated at pressures above 200 MPa, irrespective of pH (4−9), in the whole cherry tomatoes no significant inactivation was seen even after treatment at 600 MPa, presumably because other components in the tomato offered protection or the isoenzymes were different. Polygalacturonase was more susceptible to pressure, being almost totally inactivated after treatment at 500 MPa. It is concluded that the textural changes in tomato induced by pressure involve at least two related phenomena. Initially, damage is caused by the greater compressibilty of the gaseous phase (air) compared to liquid−solid components, giving rise to a compact structure which, on pressure release, is damaged as the air rapidly expands, leading to increases in membrane permeability. This permits egress of water, and the damage also enables enzymatic action to increase, causing further cell damage and softening. The major enzyme involved in the further softening is polygalacturonase, which is inactivated at 500 MPa and above, and not pectinmethylesterase, which in the whole fruit, is barotolerant. Keywords: High-pressure treatment; pectinmethylesterase; polygalacturonase; tomato; texture; structure