Self-Contained, Biomolecular Motor-Driven Protein Sorting and Concentrating in an Ultrasensitive Microfluidic Chip

We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules...

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Bibliographic Details
Published inNano letters Vol. 8; no. 4; pp. 1041 - 1046
Main Authors Lin, Chih-Ting, Kao, Ming-Tse, Kurabayashi, Katsuo, Meyhofer, Edgar
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.04.2008
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microfluidics - instrumentation
Miscellaneous
Protein Transport
Proteins
Sensitivity and Specificity
Fluid mechanics
Fluorescent material
Macromolecule
Organic molecule
Classification
Microtubule
Molecular motor
Chip
Kinesin
Microfluidics
Protein
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Summary:We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules are steered along kinesin-coated microchannel tracks toward a collector structure, concentrated, and trapped. Using fluorescent analyte molecules and nanoliter sample volumes, we demonstrated 14 fM sensitivity, even in the presence of high concentrations of other proteins.
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ISSN:1530-6984
1530-6992
DOI:10.1021/nl072742x