A Chemogenomic Toolkit to Evaluate the "Ins and Outs" of Yeast Plasma Membrane Transporters

• Published in: mBio Vol. 13; no. 3; p. e0095522
• Main Authors: Prasad, Rajendra, Banerjee, Atanu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 28.06.2022
• Subjects: chemogenomics; Commentary; double transporter gene deletion library; drug/xenobiotic transporters; high-throughput screening; Microbial Genetics; Saccharomyces cerevisiae; chemogenomics; drug/xenobiotic transporters; double transporter gene deletion library; Saccharomyces cerevisiae; high-throughput screening
• ISSN: 2150-7511; 2150-7511
• DOI: 10.1128/mbio.00955-22

Over the years, there has been a lot of emphasis on the development of high-throughput platforms that help identify transporters of drugs and xenobiotics. However, major hinderances in these approaches include substrate promiscuity and functional redundancy of membrane transporters. To tackle such issues, Almeida and colleagues (L. D. Almeida, A. S. F. Silva, D. C. Mota, A. A. Vasconcelos, et al., mBio 12(6):e03221-21, 2021) elegantly used the power of yeast genetics and created a double gene deletion library for 122 nonessential plasma membrane transporters that facilitates high-throughput identification of drug/xenobiotic transporters. While examining a library of cytotoxic compounds, the authors identified a strong correlation between the chemical structure of azoles and possible import/export routes. Interestingly, the authors also identified the -inositol transporter Itr1 to be responsible for import of triazole and imidazole antifungal compounds and proposed a role for the ABC transporter Pdr5 in carbendazim uptake.