An Integrated Microfabricated Cell Sorter

We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordina...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 74; no. 11; pp. 2451 - 2457
Main Authors Fu, Anne Y, Chou, Hou-Pu, Spence, Charles, Arnold, Frances H, Quake, Stephen R
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.06.2002
Subjects
Bacteriological Techniques
Biological and medical sciences
Cell physiology
Chemistry
Escherichia coli
Fundamental and applied biological sciences. Psychology
Instruments
Miscellaneous
Molecular and cellular biology
Nanotechnology
Cell sorter
Cell
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Summary:We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is ∼100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.
Bibliography:ark:/67375/TPS-2W6BK7DR-Q
istex:90B092A94B1E91E6480E69CD9F33139711853650
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0255330