Single Embryo-Coupled Gate Field Effect Transistor for Elective Single Embryo Transfer

In this study, we have proposed and demonstrated experimentally a novel monitoring device of single mouse embryo activity after in vitro fertilization (IVF) using a semiconductor-based field effect transistor (FET). The FET biosensor realized to detect it noninvasively, quantitatively, and continuou...

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Published inAnalytical chemistry (Washington) Vol. 85; no. 14; pp. 6633 - 6638
Main Authors Sakata, Toshiya, Saito, Akiko, Mizuno, Jinji, Sugimoto, Haruyo, Noguchi, Kaori, Kikuchi, Eiko, Inui, Hiroaki
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 16.07.2013
Subjects
Animals
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Carbon dioxide
Cellular
Devices
Embryo Culture Techniques - instrumentation
Embryo Culture Techniques - methods
Embryos
Female
Field effect transistors
In vitro fertilization
Ions
IVF
Male
Mice
Mice, Inbred ICR
Morphology
Pregnancy
Rodents
Semiconductor devices
Single Embryo Transfer - instrumentation
Single Embryo Transfer - methods
Transistors
Transistors, Electronic
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Summary:In this study, we have proposed and demonstrated experimentally a novel monitoring device of single mouse embryo activity after in vitro fertilization (IVF) using a semiconductor-based field effect transistor (FET). The FET biosensor realized to detect it noninvasively, quantitatively, and continuously by change of hydrogen ions with positive charges, which were induced by dissolved carbon dioxide due to cellular respiration activity during cleavage. The electrical signal of FET biosensor should become an effective indication to evaluate objectively single embryo activity as its morphology is observed subjectively after IVF. The platform based on the FET biosensor will contribute to promote elective single embryo transfer (eSET) in human assisted reproductive technology (ART).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/ac4001018