Solution Structure of the Complex between the B-Subunit Homopentamer of Verotoxin VT-1 from Escherichia coli and the Trisaccharide Moiety of Globotriaosylceramide

We report the solution structure of the carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic Escherichia coli in association with the trisaccharide Galα1−4Galβ1−4Glcβ1-O-trimethylsilylethyl, determined by use of stable isotope-assisted NMR techniques. In contrast to the crys...

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Published inBiochemistry (Easton) Vol. 37; no. 31; pp. 11078 - 11082
Main Authors Shimizu, H, Field, R. A, Homans, S. W, Donohue-Rolfe, A
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 04.08.1998
Subjects
Bacterial Toxins - chemistry
Bacterial Toxins - metabolism
Binding Sites
Carbohydrate Conformation
Carbon Isotopes
Escherichia coli - chemistry
Ligands
Nuclear Magnetic Resonance, Biomolecular
Protein Conformation
Shiga Toxin 1
Solutions
Trihexosylceramides - chemistry
Trihexosylceramides - metabolism
Trisaccharides - chemistry
Trisaccharides - metabolism
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Summary:We report the solution structure of the carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic Escherichia coli in association with the trisaccharide Galα1−4Galβ1−4Glcβ1-O-trimethylsilylethyl, determined by use of stable isotope-assisted NMR techniques. In contrast to the crystal structure of the complex which predicts three binding sites per monomer, only one of these sites is observed with substantial occupancy by the trisaccharide in solution.
Bibliography:This work was suppported by BBSRC Grant 49/SBD07527.
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ISSN:0006-2960
1520-4995
DOI:10.1021/bi980946+