Initial Bioadhesion on Dental Materials as a Function of Contact Time, pH, Surface Wettability, and Isoelectric Point

• Published in: Langmuir Vol. 26; no. 6; pp. 4136 - 4141
• Main Authors: Müller, Christine, Lüders, Anne, Hoth-Hannig, Wiebke, Hannig, Matthias, Ziegler, Christiane
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 16.03.2010
• Subjects: Adsorption; Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials; Chemistry; Colloidal state and disperse state; Dental Cements; Dental Materials; Exact sciences and technology; General and physical chemistry; Gold - chemistry; Hydrogen-Ion Concentration; Isoelectric Point; Polymethyl Methacrylate - chemistry; Polytetrafluoroethylene - chemistry; Serum Albumin, Bovine - chemistry; Static Electricity; Surface physical chemistry; Surface Properties; Titanium - chemistry; Wettability; Water; Gold; Bovine; Methyl methacrylate polymer; Transition metal; Serum albumin; Hydrophobicity; Adhesion; Composite material; Protein; Interface structure; Vertebrata; Wettability; Mammalia; Adsorption; Isoelectric point; Titanium; pH; Artiodactyla; Ceramic materials; Ungulata; Electrostatic force
• ISSN: 0743-7463; 1520-5827; 1520-5827
• DOI: 10.1021/la903299y

The adsorption of bovine serum albumin (BSA) on surfaces of dental enamel and of dental materials was investigated by scanning force spectroscopy. This method provides adhesion forces which can be measured as a function of contact time between protein and surface, pH, wettability, and isoelectric point of the surface. Whereas the chosen ceramic and composite materials resemble very well the adhesion on natural enamel, a much stronger adhesion was found for the more hydrophobic surfaces, that is, gold, titanium, poly(methyl methacrylate) (PMMA), and poly(tetrafluoroethylene) (PTFE). On hydrophilic surfaces, adhesion is mainly influenced by the electrostatic forces between protein and surface. However, the conformational change of BSA at pH values above pH 8 has to be taken into account. On the very hydrophobic PTFE surface, the special interface structure between PTFE and water plays an important role which governs BSA adhesion.