Super-Resolution Monitoring of Mitochondrial Dynamics upon Time-Gated Photo-Triggered Release of Nitric Oxide

Nitric oxide (NO) potentially plays a regulatory role in mitochondrial fusion and fission, which are vital to cell survival and implicated in health, disease, and aging. Molecular tools facilitating the study of the relationship between NO and mitochondrial dynamics are in need. We have recently dev...

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Published inAnalytical chemistry (Washington) Vol. 90; no. 3; pp. 2164 - 2169
Main Authors He, Haihong, Ye, Zhiwei, Xiao, Yi, Yang, Wei, Qian, Xuhong, Yang, Youjun
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 06.02.2018
Subjects
Aging
Cell survival
Cells
Chemistry
Localization
Mitochondria
Molecules
Morphology
Nitric oxide
Photoactivation
Temporal resolution
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Summary:Nitric oxide (NO) potentially plays a regulatory role in mitochondrial fusion and fission, which are vital to cell survival and implicated in health, disease, and aging. Molecular tools facilitating the study of the relationship between NO and mitochondrial dynamics are in need. We have recently developed a novel NO donor (NOD550). Upon photoactivation, NOD550 decomposes to release two NO molecules and a fluorophore. The NO release could be spatially mapped with subdiffraction resolution and with a temporal resolution of 10 s. Due to the preferential localization of NOD550 at mitochondria, morphology and dynamics of mitochondria could be monitored upon NO release from NOD550.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b04510