CwlQ Is Required for Swarming Motility but Not Flagellar Assembly in Bacillus subtilis

• Published in: Journal of bacteriology Vol. 203; no. 10; pp. 1 - 17
• Main Authors: Sanchez, Sandra, Dunn, Caroline M, Kearns, Daniel B
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 21.04.2021
• Subjects: Assembly; Bacillus subtilis; Bacteria; Bacteriology; Biosynthesis; Flagella; Gram-negative bacteria; Gram-positive bacteria; Insertion; Lytic enzymes; Macromolecules; Motility; Mutation; Osmosis; Osmotic pressure; Peptidoglycans; Research Article; Secretion; Sigma factor; Swarming; flagella; peptidoglycan; autolysin; hydrolase; swarming; motility; lytic transglycosylase
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/JB.00029-21

Lytic enzymes play an essential role in the remodeling of bacterial peptidoglycan (PG), an extracellular mesh-like structure that retains the membrane in the context of high internal osmotic pressure. Peptidoglycan must be unfailingly stable to preserve cell integrity, but must also be dynamically remodeled for the cell to grow, divide, and insert macromolecular machines. The flagellum is one such macromolecular machine that transits the PG, and flagellar insertion is aided by localized activity of a dedicated PG lyase in Gram-negative bacteria. To date, there is no known dedicated lyase in Gram-positive bacteria for the insertion of flagella. Here, we take a reverse-genetic candidate-gene approach and find that cells mutated for the lytic transglycosylase CwlQ exhibit a severe defect in flagellum-dependent swarming motility. We further show that CwlQ is expressed by the motility sigma factor SigD and is secreted by the type III secretion system housed inside the flagellum. Nonetheless, cells with mutations of CwlQ remain proficient for flagellar biosynthesis even when mutated in combination with four other lyases related to motility (LytC, LytD, LytF, and CwlO). The PG lyase (or lyases) essential for flagellar synthesis in , if any, remains unknown. Bacteria are surrounded by a wall of peptidoglycan and early work in was the first to suggest that bacteria needed to enzymatically remodel the wall to permit insertion of the flagellum. No PG remodeling enzyme alone or in combination, however, has been found to be essential for flagellar assembly in Here, we take a reverse-genetic candidate-gene approach and find that the PG lytic transglycosylase CwlQ is required for swarming motility. Subsequent characterization determined that while CwlQ was coexpressed with motility genes and is secreted by the flagellar secretion apparatus, it was not required for flagellar synthesis. The PG lyase needed for flagellar assembly in remains unknown.