Differences in Morphology and Traction Generation of Cell Lines Representing Different Stages of Osteogenesis

Osteogenesis is the process by which mesenchymal stem cells differentiate to osteoblasts and form bone. The morphology and root mean squared (RMS) traction of four cell types representing different stages of osteogenesis were quantified. Undifferentiated D1, differentiated D1, MC3T3-E1, and MLO-A5 c...

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Bibliographic Details
Published inJournal of biomechanical engineering Vol. 137; no. 12; p. 124503
Main Authors Poellmann, Michael J, Estrada, Jonathan B, Boudou, Thomas, Berent, Zachary T, Franck, Christian, Wagoner Johnson, Amy J
Format Journal Article
LanguageEnglish
Published United States 01.12.2015
Subjects
3T3 Cells
Animals
Cell Adhesion - physiology
Cell Differentiation - physiology
Cell Line
Cell Size
Computer Simulation
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Mice
Models, Biological
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - physiology
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Summary:Osteogenesis is the process by which mesenchymal stem cells differentiate to osteoblasts and form bone. The morphology and root mean squared (RMS) traction of four cell types representing different stages of osteogenesis were quantified. Undifferentiated D1, differentiated D1, MC3T3-E1, and MLO-A5 cell types were evaluated using both automated image analysis of cells stained for F-actin and by traction force microscopy (TFM). Undifferentiated mesenchymal stem cell lines were small, spindly, and exerted low traction, while differentiated osteoblasts were large, had multiple processes, and exerted higher traction. Size, shape, and traction all correlated with the differentiation stage. Thus, cell morphology evolved and RMS traction increased with differentiation. The results provide a foundation for further work with these cell lines to study the mechanobiology of bone formation.
ISSN:1528-8951
DOI:10.1115/1.4031848