Dissecting Sperm Mitochondrial G‑Quadruplex Structures Using a Fluorescent Probe Biomarker to Monitor and Regulate Fertilization Capability

To monitor the levels of mitochondrial DNA G-quadruplexes (mtDNA G4s) in spermatozoa and to explore the possibility using mtDNA G4s as a reliable marker in patients with multiple clinical insemination failures, a novel chemical TPE-mTO probe engineered in our previous work was used on both samples f...

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Published inACS sensors Vol. 8; no. 6; pp. 2186 - 2196
Main Authors Wu, Sixian, Li, Xiaoliang, Huang, Gelin, Guo, Juncen, Zhang, Xueguang, Liao, Lu, Zhi, Weiwei, Li, Kun, Wang, Yan, Chu, Zhiwen, Shang, Lijun, Yu, Xiaoqi, Yu, Kangkang, Xu, Wenming
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 23.06.2023
Subjects
Animals
Cricetinae
DNA, Mitochondrial - genetics
DNA, Mitochondrial - metabolism
Fluorescent Dyes - metabolism
G-Quadruplexes
Humans
Male
Mice
Semen
Sperm-Ovum Interactions
Spermatozoa - metabolism
mitophagy
G-quadruplexes
spermatozoa
fertilization failure
mitochondria
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Summary:To monitor the levels of mitochondrial DNA G-quadruplexes (mtDNA G4s) in spermatozoa and to explore the possibility using mtDNA G4s as a reliable marker in patients with multiple clinical insemination failures, a novel chemical TPE-mTO probe engineered in our previous work was used on both samples from the mice sperm and from patients with fertilization failure. Expression of valosin-containing protein and the zona-free hamster egg assay were used to evaluate mitophagy and human sperm penetration. RNA-sequencing was used to explore expression changes of key genes affected by mtDNA G4s. Results showed that the probe can track mtDNA G4s in spermatozoa easily and quickly with fewer backgrounds. Significantly increased mtDNA G4s were also found in patients with fertilization failure, using the flow-cytometry-based TPE-mTO probe detection method. A sperm–hamster egg penetration experiment showed that abnormal fertilization caused by increased mtDNA G4s can be effectively restored by a mitophagy inducer. This study provides a novel method for monitoring etiological biomarkers in patients with clinical infertility and treatment for patients with abnormal fertilization caused by mtDNA G4 dysfunction.
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ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.3c00068