Bioelectrochemical Monitoring of Phenols and Aromatic Amines in Flow Injection Using Novel Plant Peroxidases

• Published in: Analytical chemistry (Washington) Vol. 70; no. 13; pp. 2596 - 2600
• Main Authors: Munteanu, Florentina-Daniela, Lindgren, Annika, Emnéus, Jenny, Gorton, Lo, Ruzgas, Tautgirdas, Csöregi, Elisabeth, Ciucu, Anton, van Huystee, R. B, Gazaryan, Irina G, Lagrimini, L. Mark
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.07.1998
• Subjects: Aniline Compounds - analysis; Animals; Arachis - enzymology; Aspergillus niger - enzymology; Biosensing Techniques; Carbohydrate Epimerases; Chemistry; Flow Injection Analysis; Glucose Oxidase; Horseradish Peroxidase; Kidney - enzymology; Nicotiana - enzymology; Phenols - analysis; Plants, Genetically Modified; Plants, Toxic; Stereoisomerism; Swine
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac980022s

An amperometric flow system combined with a glucose oxidase−mutarotase reactor was optimized and used to determine aromatic amines and phenols using peroxidase-modified graphite electrodes. An increase in currents upon injection of the analyzed substrate was shown to be approximated by a Michaelis−Menten type dependence. The detection limit was calculated as 3 times the noise, and the sensitivity was calculated as I max/ . Commercially available horseradish peroxidase was compared with tobacco anionic and peanut cationic peroxidases for determination of aromatic amines and phenols. Detection limits of 10 nM for determination of o-aminophenol and o- and p-phenylenediamine achieved with a tobacco peroxidase-modified electrode give a promise for further improvements in sensitivities and detection limits of biosensors.